Evidence map›Paper›PMID 38907145›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

A Sample Preparation Procedure for Isobaric Labeling-Based Single-Cell Proteomics.

Consuelo Marín-Vicente, Enrique Calvo, José Manuel Rodríguez, Cristina Villa Del Campo, Rocío Sierra, Ákos Végvári, Roman A Zubarev, Miguel Torres, Jesús Vázquez

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Consuelo Marín-VicenteCardiovascular Proteomics Group, Spanish National Centre for Cardiovascular Research (CNIC), Madrid, Spain. cmarin@cnic.es.
Enrique CalvoProteomics Unit, Spanish National Centre for Cardiovascular Research (CNIC), Madrid, Spain.
José Manuel RodríguezProteomics Unit, Spanish National Centre for Cardiovascular Research (CNIC), Madrid, Spain.
Cristina Villa Del CampoGenetic Control of Development and Organ Regeneration Group, Spanish National Centre for Cardiovascular Research (CNIC), Madrid, Spain.
Rocío SierraGenetic Control of Development and Organ Regeneration Group, Spanish National Centre for Cardiovascular Research (CNIC), Madrid, Spain.
Ákos VégváriDivision of Chemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Roman A ZubarevDivision of Chemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Miguel TorresGenetic Control of Development and Organ Regeneration Group, Spanish National Centre for Cardiovascular Research (CNIC), Madrid, Spain. mtorres@cnic.es.
Jesús VázquezCardiovascular Proteomics Group, Spanish National Centre for Cardiovascular Research (CNIC), Madrid, Spain. jesus.vazquez@cnic.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mass spectrometry-based proteomics has traditionally been limited by the amount of input material for analysis. Single-cell proteomics has emerged as a challenging discipline due to the ultra-high sensitivity required. Isobaric labeling-based multiplex strategies with a carrier proteome offer an approach to overcome the sensitivity limitations. Following this as the basic strategy, we show here the general workflow for preparing cells for single-cell mass spectrometry-based proteomics. This protocol can also be applied to manually isolated cells when large cells, such as cardiomyocytes, are difficult to isolate properly with conventional fluorescence-activated cell sorting (FACS) sorter methods.

Indexed as

ProteomicsSingle-Cell AnalysisAnimalsFlow CytometryHumansIsotope LabelingMass SpectrometryMyocytes, CardiacProteomeStaining and LabelingProteomeMass spectrometryMultiplex sample preparation strategyOrbitrap Eclipse mass spectrometerSingle-cell proteomicsTandem mass tagTribrid mass spectrometer

Identifiers

PMID38907145

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.