Infrared Laser Ablation and Capture of Biological Tissue.

Blessing C Egbejiogu, Fabrizio Donnarumma, Chao Dong, Kermit K Murray

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Are Native Conformations of Proteins Retained Throughout Laser Ablation Capture?Analytical chemistry · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Blessing C EgbejioguDepartment of Chemistry, Louisiana State University, Baton Rouge, LA, USA.

Fabrizio DonnarummaDepartment of Chemistry, Louisiana State University, Baton Rouge, LA, USA.

Chao DongDepartment of Chemistry, Louisiana State University, Baton Rouge, LA, USA.

Kermit K MurrayDepartment of Chemistry, Louisiana State University, Baton Rouge, LA, USA. kkmurray@lsu.edu.

Funding

National Science Foundation CHE-1709526

6 · The paper itself

Abstract

Sampling thin tissue sections with cellular precision can be accomplished using laser ablation microsampling for mass spectrometry analysis. In this work, the use of a pulsed mid-infrared (IR) laser for selecting small regions of interest (ROI) in tissue sections for offline liquid chromatography-tandem mass spectrometry (LC-MS/MS) is described. The laser is focused onto the tissue section, which is rastered as the laser is fired. The ablated tissue is captured in a microwell array and processed in situ through reduction, alkylation, and digestion with a low liquid volume workflow. The resulting peptides from areas as small as 0.01 mm

Indexed as

Laser TherapyTandem Mass SpectrometryAnimalsChromatography, LiquidHumansInfrared RaysLasersProteomicsLaser ablationMicrosamplingProteomics

Identifiers

PMID38907143

DOI10.1038/nmeth1094

What OpenQuestion holds

Textmetadata

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.