Evidence map›Paper›PMID 38906490›Full record

ArticleReproductive toxicology (Elmsford, N.Y.)2024

Rapid identification of reproductive toxicants among environmental chemicals using an in vivo evaluation of gametogenesis in budding yeast Saccharomyces cerevisiae.

Ravinder Kumar, Ashwini Oke, Beth Rockmill, Matthew de Cruz, Rafael Verduzco, Anura Shodhan, Xavier Woodruff-Madeira, Dimitri P Abrahamsson, Julia Varshavsky, Juleen Lam and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive toxicology (Elmsford, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. FIGO committee opinion: Environmental drivers of gynecologic and reproductive health.International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics · 2025
    Article
  2. FIGO committee opinion: Environmental drivers of obstetric health and early childhood development.International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics · 2025
    Article
  3. Article
  4. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Ravinder KumarDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA; Center of Reproductive Sciences, University of California, San Francisco, CA, USA.
Ashwini OkeDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA; Center of Reproductive Sciences, University of California, San Francisco, CA, USA.
Beth RockmillDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA; Center of Reproductive Sciences, University of California, San Francisco, CA, USA.
Matthew de CruzDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA; Center of Reproductive Sciences, University of California, San Francisco, CA, USA.
Rafael VerduzcoDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA; Center of Reproductive Sciences, University of California, San Francisco, CA, USA.
Anura ShodhanDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA; Center of Reproductive Sciences, University of California, San Francisco, CA, USA.
Xavier Woodruff-MadeiraDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA; Center of Reproductive Sciences, University of California, San Francisco, CA, USA.
Dimitri P AbrahamssonDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA.
Julia VarshavskyDepartment of Health Sciences and Department of Civil and Environmental Engineering, Northeastern University, Boston, MA, USA.
Juleen LamDepartment of Public Health, California State University, East Bay, Hayward, CA, USA.
Joshua F RobinsonDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA; Center of Reproductive Sciences, University of California, San Francisco, CA, USA.
Patrick AllardUCLA Institute for Society & Genetics, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA.
Tracey J WoodruffDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA.
Jennifer C FungDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA, USA; Center of Reproductive Sciences, University of California, San Francisco, CA, USA. Electronic address: jennifer.fung@ucsf.edu.

Funding

UCSF Environmental Research and Translation for Health Center (EaRTH Center)P30ES030284 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JENNIFER C FUNG, Tracey J. Woodruff · 2020 to 2026
$11.2M
Transdisciplinary Approach to Rapidly Identify Reproductive Toxics in Pregnant Women and NewbornsR01ES027051 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Dimitri Abrahamsson, Tracey J. Woodruff · 2016 to 2026
$6.6M
Quantitative Analysis of Meiotic Chromosome Motion and PairingR01GM137126 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FUNG, JENNIFER C · 2020 to 2023
$1.5M
Advancing chemical risk assessment through development, validation and integration of high throughput mechanistic in vitro dataR15ES033816 · NIEHS · CALIFORNIA STATE UNIVERSITY HAYWARD · PI LAM, JULEEN · 2022 to 2022
$462k
IN VITRO SPERMATOGENIC CELL GENOTOXICITY TESTSR01ES003381 · NIEHS · WEILL MEDICAL COLLEGE OF CORNELL UNIV · PI RISLEY, MICHAEL S · 1985 to 1989
–
NIEHS NIH HHS P30 ES030284NIEHS NIH HHS R01 ES027051NIEHS NIH HHS R15 ES033816NIGMS NIH HHS R01 GM137126NIH HHS 1R15ES033816–01A1NIH HHS P30ES030284NIH HHS R01ES027051 02S1NIH HHS R01GM137126
6 · The paper itself

Abstract

Infertility affects ∼12 % of couples, with environmental chemical exposure as a potential contributor. Of the chemicals that are actively manufactured, very few are assessed for reproductive health effects. Rodents are commonly used to evaluate reproductive effects, which is both costly and time consuming. Thus, there is a pressing need for rapid methods to test a broader range of chemicals. Here, we developed a strategy to evaluate large numbers of chemicals for reproductive toxicity via a yeast, S. cerevisiae high-throughput assay to assess gametogenesis as a potential new approach method (NAM). By simultaneously assessing chemicals for growth effects, we can distinguish if a chemical affects gametogenesis only, proliferative growth only or both. We identified a well-known mammalian reproductive toxicant, bisphenol A (BPA) and ranked 19 BPA analogs for reproductive harm. By testing mixtures of BPA and its analogs, we found that BPE and 17 β-estradiol each together with BPA showed synergistic effects that worsened reproductive outcome. We examined an additional 179 environmental chemicals including phthalates, pesticides, quaternary ammonium compounds and per- and polyfluoroalkyl substances and found 57 with reproductive effects. Many of the chemicals were found to be strong reproductive toxicants that have yet to be tested in mammals. Chemicals having affect before meiosis I division vs. meiosis II division were identified for 16 gametogenesis-specific chemicals. Finally, we demonstrate that in general yeast reproductive toxicity correlates well with published reproductive toxicity in mammals illustrating the promise of this NAM to quickly assess chemicals to prioritize the evaluation for human reproductive harm.

Indexed as

Benzhydryl CompoundsEnvironmental PollutantsGametogenesisPhenolsSaccharomyces cerevisiaeAnimalsBisphenol A CompoundsEndocrine DisruptorsEstradiolHigh-Throughput Screening AssaysReproductionToxicity TestsBenzhydryl Compoundsbisphenol ABisphenol A CompoundsEndocrine DisruptorsEnvironmental PollutantsEstradiolPhenolsbisphenolsgametogenesishigh-throughputinfertilitymeiosisNAMquaternary ammonium compoundsreproductive toxicity

Identifiers

PMID38906490
PMCPMC11648579

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.