ArticleScience advances2024
Freestanding bilayer microscope for single-molecule imaging of membrane proteins.
Article in Science advances, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Spatial Deconstruction of the Plasma Membrane.Journal of the American Chemical Society · 2026Article
- Biomolecular assemblies through weak noncovalent interactions: Higher-order transient structures and their condensate phase.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Accurate Single-Particle Tracking and Diffusion Measurement in Freestanding Lipid Bilayers and Model Membranes.Analytical chemistry · 2025Article
- Article
- Visualizing PIEZO1 localization and activity in hiPSC-derived single cells and organoids with HaloTag technology.Nature communications · 2025Article
- Advancements in Single-Molecule Fluorescence Detection Techniques and Their Expansive Applications in Drug Discovery and Neuroscience.Biosensors · 2025Review
- Visualizing PIEZO1 Localization and Activity in hiPSC-Derived Single Cells and Organoids with HaloTag Technology.bioRxiv : the preprint server for biology · 2025Article
- Advancing membrane biology: single-molecule approaches meet model membrane systems.BMB reports · 2025Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Integral membrane proteins (IMPs) constitute a large fraction of organismal proteomes, playing fundamental roles in physiology and disease. Despite their importance, the mechanisms underlying dynamic features of IMPs, such as anomalous diffusion, protein-protein interactions, and protein clustering, remain largely unknown due to the high complexity of cell membrane environments. Available methods for in vitro studies are insufficient to study IMP dynamics systematically. This publication introduces the freestanding bilayer microscope (FBM), which combines the advantages of freestanding bilayers with single-particle tracking. The FBM, based on planar lipid bilayers, enables the study of IMP dynamics with single-molecule resolution and unconstrained diffusion. This paper benchmarks the FBM against total internal reflection fluorescence imaging on supported bilayers and is used here to estimate ion channel open probability and to examine the diffusion behavior of an ion channel in phase-separated bilayers. The FBM emerges as a powerful tool to examine membrane protein/lipid organization and dynamics to understand cell membrane processes.
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Registered trials
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