Evidence map›Paper›PMID 38904915›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Isolation, Characterization, and Utilization of Human Skin Basal and Suprabasal Epidermal Stem Cells.

Luca Pontiggia, Agnes S Klar, Katarzyna Michalak-Micka, Ueli Moehrlen, Thomas Biedermann

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Luca PontiggiaTissue Biology Research Unit, Department of Pediatric Surgery, University Children's Hospital Zurich, Schlieren, Switzerland.
Agnes S KlarTissue Biology Research Unit, Department of Pediatric Surgery, University Children's Hospital Zurich, Schlieren, Switzerland.
Katarzyna Michalak-MickaTissue Biology Research Unit, Department of Pediatric Surgery, University Children's Hospital Zurich, Schlieren, Switzerland.
Ueli MoehrlenTissue Biology Research Unit, Department of Pediatric Surgery, University Children's Hospital Zurich, Schlieren, Switzerland.
Thomas BiedermannTissue Biology Research Unit, Department of Pediatric Surgery, University Children's Hospital Zurich, Schlieren, Switzerland. thomas.biedermann@kispi.uzh.ch.

Funding

Swiss National Science Foundation 310030_207793
6 · The paper itself

Abstract

Studying human skin biology can aid in comprehending the pathophysiology of skin diseases and developing novel cell-based therapies, including tissue engineering approaches. This chapter provides a comprehensive guide of methods to determine human skin samples from the perspective of their cellular compositions. We describe as useful technique the histological analysis of tissue sections. We further illustrate the biological characterization of isolated and cultured basal and suprabasal interfollicular keratinocytes by cell sorting, cytospin immunostaining, colony forming efficiency, and long-term dermo-epidermal organotypic cultures.

Indexed as

Cell SeparationEpidermal CellsKeratinocytesStem CellsCell Culture TechniquesCell DifferentiationCells, CulturedEpidermisHumansSkinTissue EngineeringHomeostasisIntegrin beta 4Interfollicular epidermisKeratinocyteRegenerationStem cells

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.