Evidence map›Paper›PMID 38903095›Full record

ArticlebioRxiv : the preprint server for biology2024

Kif1a and intact microtubules maintain synaptic-vesicle populations at ribbon synapses in zebrafish hair cells.

Sandeep David, Katherine Pinter, Keziah-Khue Nguyen, David S Lee, Zhengchang Lei, Yuliya Sokolova, Lavinia Sheets, Katie S Kindt

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Sandeep DavidSection on Sensory Cell Development and Function, National Institute on Deafness and other Communication Disorders, Bethesda, MD, USA.
Katherine PinterSection on Sensory Cell Development and Function, National Institute on Deafness and other Communication Disorders, Bethesda, MD, USA.
Keziah-Khue NguyenDepartment of Otolaryngology - Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
David S LeeDepartment of Otolaryngology - Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Zhengchang LeiSection on Sensory Cell Development and Function, National Institute on Deafness and other Communication Disorders, Bethesda, MD, USA.
Yuliya SokolovaAdvanced Imaging Core, National Institute on Deafness and other Communication Disorders, Bethesda, MD, USA.
Lavinia SheetsDepartment of Otolaryngology - Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.ORCID 0000-0001-5231-2450
Katie S KindtSection on Sensory Cell Development and Function, National Institute on Deafness and other Communication Disorders, Bethesda, MD, USA.ORCID 0000-0002-1065-8215

Funding

In vivo investigation of sensory hair cell function and developmentZIADC000085 · NIDCD · NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS · PI KINDT, KATIE · 2014 to 2025
$21.4M
Advanced Imaging CoreZICDC000081 · NIDCD · NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS · PI WINKLER, DENNIS · 2012 to 2025
$17.4M
DEVELOPMENT OF CLINICIAN/RESEARCHERS IN ACADEMIC ENTT32DC000022 · NIDCD · WASHINGTON UNIVERSITY · PI PICCIRILLO, JAY F. · 1989 to 2022
$5.6M
Roles of the Synapse in Hair-Cell PathologyR01DC016066 · NIDCD · WASHINGTON UNIVERSITY · PI Lavinia Sheets · 2017 to 2026
$3.6M
Intramural NIH HHS ZIA DC000085Intramural NIH HHS ZIC DC000081NIDCD NIH HHS R01 DC016066NIDCD NIH HHS T32 DC000022
6 · The paper itself

Abstract

Sensory hair cells of the inner ear utilize specialized ribbon synapses to transmit sensory stimuli to the central nervous system. This sensory transmission necessitates rapid and sustained neurotransmitter release, which relies on a large pool of synaptic vesicles at the hair-cell presynapse. Work in neurons has shown that kinesin motor proteins traffic synaptic material along microtubules to the presynapse, but how new synaptic material reaches the presynapse in hair cells is not known. We show that the kinesin motor protein Kif1a and an intact microtubule network are necessary to enrich synaptic vesicles at the presynapse in hair cells. We use genetics and pharmacology to disrupt Kif1a function and impair microtubule networks in hair cells of the zebrafish lateral-line system. We find that these manipulations decrease synaptic-vesicle populations at the presynapse in hair cells. Using electron microscopy, along with

Indexed as

hair cellskinesinsRibbon synapsessensory systemssynaptic vesicleszebrafish

Identifiers

PMID38903095
PMCPMC11188139

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.