Evidence map›Paper›PMID 38900499›Full record

ArticleJournal of proteome research2024

Electron Capture vs Transfer Dissociation for Site Determination of Tryptic Peptide Tyrosine Sulfation: Direct Detection of Fibrinogen Sulfation Sites and Identification of Novel Isobaric Interferences.

Menatallah M Youssef, Carson W Szot, Jeff Folz, Luke M Collier, Hye Kyong Kweon, Steven A DeFiglia, Miriam F Ayad, Lobna A Hussein, Maha F Abdel-Ghany, Kristina Hakansson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of proteome research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Menatallah M YoussefDepartment of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055, United States.
Carson W SzotDepartment of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055, United States.
Jeff FolzDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109-1055, United States.ORCID 0000-0002-4823-3343
Luke M CollierDepartment of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055, United States.ORCID 0009-0003-7950-6927
Hye Kyong KweonDepartment of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055, United States.
Steven A DeFigliaDepartment of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055, United States.
Miriam F AyadDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt.
Lobna A HusseinDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt.
Maha F Abdel-GhanyDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt.
Kristina HakanssonDepartment of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055, United States.ORCID 0000-0003-1926-0542

Funding

Enabling Mass Spectrometry Analysis of the Sulfoproteome SupplementR01GM139916 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAKANSSON, KRISTINA · 2021 to 2024
$1.4M
Orbitrap Fusion Lumos ETD with IRMPD for UM Chemistry MS Facility ExpansionS10OD021619 · OD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAKANSSON, KRISTINA · 2016 to 2016
$1.1M
NIGMS NIH HHS R01 GM139916NIH HHS S10 OD021619
6 · The paper itself

Abstract

Tyrosine sulfation, an understudied but crucial post-translational modification, cannot be directly detected in conventional nanoflow liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) due to the extreme sulfate lability. Here, we report the detection of sulfate-retaining fragments from LC-electron capture dissociation (ECD) and nanoLC-electron transfer higher energy collision dissociation (EThcD). Sulfopeptide candidates were identified by Proteome Discoverer and MSFragger analysis of nanoLC-HCD MS/MS data and added to inclusion lists for LC-ECD or nanoLC-EThcD MS/MS. When this approach failed, targeted LC-ECD with fixed

Indexed as

FibrinogenTandem Mass SpectrometryTyrosineAmino Acid SequenceChromatography, LiquidElectronsHumansPeptidesProtein Processing, Post-TranslationalSulfatesTrypsinFibrinogenPeptidesSulfatesTrypsinTyrosinecysteinyl-glycinedirect PTM determinationelectron capture dissociationelectron transfer dissociationpost-translational modificationsproteomicssite localization, mass spectrometrytyrosine sulfation

Identifiers

PMID38900499
PMCPMC11231624

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.