Evidence map›Paper›PMID 38900400›Full record

ArticleApplied biochemistry and biotechnology2024

Enhancing L-asparagine Production Through In Vivo ATP Regeneration System Utilizing Glucose Metabolism of Escherichia coli.

Yucheng Fan, Zijia Wei, Yuhua Zhang, Xuguo Duan

Abstract read
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In one paragraph

Article in Applied biochemistry and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yucheng FanCollege of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China.
Zijia WeiCollege of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China.
Yuhua ZhangCollege of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China.
Xuguo DuanCollege of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China. xgduan@njfu.edu.cn.ORCID http://orcid.org/0000-0002-2136-0449

Funding

a grant from Nanjing Forestry University GXL2018010University-Industry Cooperation Research Project in Jiangsu BY2022806
6 · The paper itself

Abstract

L-asparaginase synthetase, an ATP-dependent enzyme, necessitates ATP for its catalytic activity. However, the integration of L-asparaginase synthetase into industrial processes is curtailed by the prohibitive cost of ATP. To address this limitation, this study explores the construction of an efficient ATP regeneration system using the glucose metabolism of Escherichia coli, synergistically coupled with L-asparaginase synthetase catalysis. The optimal conditions for L-asparagine yield were determined in shake flasks. A total of 2.7 g/L was the highest yield achieved under specific parameters, including 0.1 mol/L of substrate, 0.2 mol/L glucose, 0.01 mol/L MgCl

Indexed as

Adenosine TriphosphateAsparagineEscherichia coliGlucoseFermentationAdenosine TriphosphateAsparagineGlucoseATP regenerationEscherichia coliL-asparagineTransformation optimization

Identifiers

PMID38900400

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.