Multiplex PCR for Early Generation Identification of Tomato Segregants Carrying Ty-2, Ty-3 and Ph-3 Resistance Alleles Against Leaf Curl and Late Blight Diseases.

Vijaya Lakshmi, Awnish Kumar, Surabhi Sangam, Shirin Akhtar, Tirthartha Chattopadhyay

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Article in Molecular biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Vijaya Lakshmi *Department of Plant Breeding and Genetics, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813210, India.ORCID http://orcid.org/0009-0004-2179-0555

Awnish Kumar *Department of Plant Breeding and Genetics, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813210, India.ORCID http://orcid.org/0000-0002-2607-9779

Surabhi SangamDepartment of Horticulture (Vegetable and Floriculture), Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813210, India.ORCID http://orcid.org/0000-0001-6132-439X

Shirin AkhtarDepartment of Horticulture (Vegetable and Floriculture), Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813210, India.ORCID http://orcid.org/0000-0002-8137-9467

Tirthartha ChattopadhyayDepartment of Plant Breeding and Genetics, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, 813210, India. tirthartha@gmail.com.ORCID http://orcid.org/0000-0001-5654-0303

Funding

Bihar Agricultural University SNP/CI/Rabi/2018-5

6 · The paper itself

Abstract

Deployment of different natural disease resistance alleles is the most sustainable and eco-friendly way for multiple disease management in tomato. Diagnostic molecular markers are indispensible in this effort as they offer early generation identification of resistance alleles in an environment-independent manner. Moreover, optimized multiplex polymerase chain reaction (PCR) for detecting different disease resistance alleles in a single reaction can speed-up the selection process with cost and labour-effectiveness. Here we report the optimized multiplex detection and stacking of leaf curl disease resistance alleles Ty-2 and Ty-3 along with late blight disease resistance allele Ph-3 in tomato genotypes and F

Indexed as

Disease ResistanceMultiplex Polymerase Chain ReactionPlant DiseasesSolanum lycopersicumAllelesGenotypeBegomovirusDisease resistance allelesFruit qualityMarker assisted gene pyramidingMultiplex PCRPhytophthora

Identifiers

PMID38900362

DOI10.1007/s12033-024-01220-8

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.