ArticleProceedings of the National Academy of Sciences of the United States of America1985
Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 139 papers.
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Who cites it
139 citing papers in PubMed.
- Cadmium binding by the F-box domain induces p97-mediated SCF complex disassembly to activate stress response programs.Nature communications · 2024Article
- Gradient tracking in mating yeast depends on Bud1 inactivation and actin-independent vesicle delivery.The Journal of cell biology · 2022Article
- Cdc48 cofactor Shp1 regulates signal-induced SCFProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Haspin regulates Ras localization to promote Cdc24-driven mitotic depolarization.Cell discovery · 2020Article
- Mating yeast cells use an intrinsic polarity site to assemble a pheromone-gradient tracking machine.The Journal of cell biology · 2019Article
- Comprehensive and quantitative analysis of G1 cyclins. A tool for studying the cell cycle.PloS one · 2019Article
- A Journey in Science: Cell-Cycle Control.Molecular medicine (Cambridge, Mass.) · 2017Article
- Myo2p is the major motor involved in actomyosin ring contraction in fission yeast.Current biology : CB · 2017Article
- Gβ promotes pheromone receptor polarization and yeast chemotropism by inhibiting receptor phosphorylation.Science signaling · 2016Article
- Skp1 Independent Function of Cdc53/Cul1 in F-box Protein Homeostasis.PLoS genetics · 2015Article
- Experimental testing of a new integrated model of the budding yeast Start transition.Molecular biology of the cell · 2015Article
- Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis.Open biology · 2014Article
- Cks1 enhances transcription efficiency at the GAL1 locus by linking the Paf1 complex to the 19S proteasome.Eukaryotic cell · 2013Article
- Signal-induced disassembly of the SCF ubiquitin ligase complex by Cdc48/p97.Molecular cell · 2012Article
- New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomycescerevisiae.G3 (Bethesda, Md.) · 2012Article
- Double-negative feedback between S-phase cyclin-CDK and CKI generates abruptness in the G1/S switch.Frontiers in physiology · 2012Article
- Coordinated regulation of sulfur and phospholipid metabolism reflects the importance of methylation in the growth of yeast.Molecular biology of the cell · 2011Article
- Function following form: functional differentiation of mammary epithelial cells requires laminin-induced polarization of PI3-kinase.Cell cycle (Georgetown, Tex.) · 2011Article
- A transcriptional activator is part of an SCF ubiquitin ligase to control degradation of its cofactors.Molecular cell · 2010Article
- Dse1 may control cross talk between the pheromone and filamentation pathways in yeast.Current genetics · 2009Article
79 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
Abstract
Antibodies raised against the protein encoded by a lacZ-CDC28 in-frame fusion were shown to immunoprecipitate the CDC28 product from yeast cell lysates. The polypeptide p36CDC28 is a phosphoprotein of apparent Mr 36,000. Immune complexes prepared from yeast cell lysates by using anti-CDC28 antibody were found to possess a protein kinase activity, as determined by the transfer of label from [gamma-32P]ATP to a coprecipitated Mr 40,000 protein of unknown identity or function (p40). This activity was absent or thermolabile when extracts were prepared from several different cdc28 temperature-sensitive strains. The protein kinase activity was dependent on Zn2+ and transferred phosphate specifically to serine and threonine residues.
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