Evidence map›Paper›PMID 38898365›Full record

ArticleIn vitro cellular & developmental biology. Animal2024

A modified method for isolating sinoatrial node myocytes from adult mice.

Qiang Li, Hanying Zhang, Ronghua Liu, Luqi Wang, Xintong Guo, Hongjie You, Jingyi Xue, Dali Luo

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Article in In vitro cellular & developmental biology. Animal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Qiang LiDepartment of Pharmacology, School of Basic Medical Sciences, Beijing Key Laboratory of Metabolic Disturbance Related Cardiovascular Disease, Capital Medical University, Beijing, 100069, People's Republic of China.
Hanying ZhangDepartment of Pharmacology, School of Basic Medical Sciences, Beijing Key Laboratory of Metabolic Disturbance Related Cardiovascular Disease, Capital Medical University, Beijing, 100069, People's Republic of China.
Ronghua LiuDepartment of Pharmacology, School of Basic Medical Sciences, Beijing Key Laboratory of Metabolic Disturbance Related Cardiovascular Disease, Capital Medical University, Beijing, 100069, People's Republic of China.
Luqi WangDepartment of Pharmacology, School of Basic Medical Sciences, Beijing Key Laboratory of Metabolic Disturbance Related Cardiovascular Disease, Capital Medical University, Beijing, 100069, People's Republic of China.
Xintong GuoDepartment of Pharmacology, School of Basic Medical Sciences, Beijing Key Laboratory of Metabolic Disturbance Related Cardiovascular Disease, Capital Medical University, Beijing, 100069, People's Republic of China.
Hongjie YouDepartment of Pharmacology, School of Basic Medical Sciences, Beijing Key Laboratory of Metabolic Disturbance Related Cardiovascular Disease, Capital Medical University, Beijing, 100069, People's Republic of China.
Jingyi XueDepartment of Pharmacology, School of Basic Medical Sciences, Beijing Key Laboratory of Metabolic Disturbance Related Cardiovascular Disease, Capital Medical University, Beijing, 100069, People's Republic of China. xuejingyi@ccmu.edu.cn.ORCID http://orcid.org/0009-0009-7305-3262
Dali LuoDepartment of Pharmacology, School of Basic Medical Sciences, Beijing Key Laboratory of Metabolic Disturbance Related Cardiovascular Disease, Capital Medical University, Beijing, 100069, People's Republic of China. luodl@ccmu.edu.cn.

Funding

National Natural Science Foundation of China 81570206National Natural Science Foundation of China 81970197National Natural Science Foundation of China 82170319
6 · The paper itself

Abstract

Sinoatrial node (SAN) is the pacemaker of the heart in charge of initiating spontaneous electronical activity and controlling heart rate. Myocytes from SAN can generate spontaneous rhythmic action potentials, which propagate through the myocardium, thereby triggering cardiac myocyte contraction. Acutely, the method for isolating sinoatrial node myocytes (SAMs) is critical in studying the protein expression and function of myocytes in SAN. Currently, the SAMs were isolated by transferring SAN tissue directly into the digestion solution, but it is difficult to judge the degree of digestion, and the system was unstable. Here, we present a modified protocol for the isolation of SAMs in mice, based on the collagenase II and protease perfusion of the heart using a Langendorff apparatus and subsequent dissociation of SAMs. The appearance and droplet flow rate of the heart could be significantly changed during enzymatic digestion via perfusion, which allowed us to easily judge the degree of digestion and avoid incomplete or excessive digestion. The SAMs with stable yield and viability achieved from our optimized approach would facilitate the follow-up experiments.

Indexed as

Cell SeparationMyocytes, CardiacSinoatrial NodeAnimalsMaleMiceMice, Inbred C57BLPerfusionLangendorff perfusionMouse sinoatrial node myocytesMurine heartPrimary cell isolation

Identifiers

PMID38898365

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.