Evidence map›Paper›PMID 38896639›Full record

ArticleJournal of applied oral science : revista FOB2024

The effect of injectable platelet-rich fibrin and platelet-rich fibrin in regenerative endodontics: a comparative in vitro study.

Jing Pan, Linjuan Luo, Zhen Jiang, Haiyan Huang, Beizhan Jiang

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of applied oral science : revista FOB, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Cell homing within endodontic hydrogels: A scoping review.The Japanese dental science review · 2026
    Review
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  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jing PanShanghai Engineering Research Center of Tooth Restoration and Regeneration , Stomatological Hospital and Dental School of Tongji University , Department of Pediatric Dentistry, Shanghai , China .
Linjuan LuoShanghai Engineering Research Center of Tooth Restoration and Regeneration , Stomatological Hospital and Dental School of Tongji University , Department of Pediatric Dentistry, Shanghai , China .
Zhen JiangShanghai Engineering Research Center of Tooth Restoration and Regeneration , Stomatological Hospital and Dental School of Tongji University , Department of Pediatric Dentistry, Shanghai , China .
Haiyan HuangShanghai Engineering Research Center of Tooth Restoration and Regeneration , Stomatological Hospital and Dental School of Tongji University , Department of Pediatric Dentistry, Shanghai , China .
Beizhan JiangShanghai Engineering Research Center of Tooth Restoration and Regeneration , Stomatological Hospital and Dental School of Tongji University , Department of Pediatric Dentistry, Shanghai , China .ORCID http://orcid.org/0000-0002-0135-5917

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo explore the feasibility of injectable platelet-rich fibrin (i-PRF) in regenerative endodontics by comparing the effect of i-PRF and platelet-rich fibrin (PRF) on the biological behavior and angiogenesis of human stem cells from the apical papilla (SCAPs). METHODOLOGY: i-PRF and PRF were obtained from venous blood by two different centrifugation methods, followed by hematoxylin-eosin (HE) staining and scanning electron microscopy (SEM). Enzyme-linked immunosorbent assay (ELISA) was conducted to quantify the growth factors. SCAPs were cultured with different concentrations of i-PRF extract (i-PRFe) and PRF extract (PRFe), and the optimal concentrations were selected using the Cell Counting Kit-8 (CCK-8) assay. The cell proliferation and migration potentials of SCAPs were then observed using the CCK-8 and Transwell assays. Mineralization ability was detected by alizarin red staining (ARS), and angiogenesis ability was detected by tube formation assay. Real-time quantitative polymerase chain reaction (RT-qPCR) was performed to evaluate the expression of genes related to mineralization and angiogenesis. The data were subjected to statistical analysis.

resultsi-PRF and PRF showed a similar three-dimensional fibrin structure, while i-PRF released a higher concentration of growth factors than PRF ( P <.05). 1/4× i-PRFe and 1/4× PRFe were selected as the optimal concentrations. The cell proliferation rate of the i-PRFe group was higher than that of the PRFe group ( P <.05), while no statistical difference was observed between them in terms of cell mitigation ( P >.05). More importantly, our results showed that i-PRFe had a stronger effect on SCAPs than PRFe in facilitating mineralization and angiogenesis, with the consistent result of RT-qPCR ( P <.05).

conclusionThis study revealed that i-PRF released a higher concentration of growth factors and was superior to PRF in promoting proliferation, mineralization and angiogenesis of SCAPs, which indicates that i-PRF could be a promising biological scaffold for application in pulp regeneration.

Indexed as

Cell ProliferationEnzyme-Linked Immunosorbent AssayIntercellular Signaling Peptides and ProteinsMicroscopy, Electron, ScanningNeovascularization, PhysiologicPlatelet-Rich FibrinReal-Time Polymerase Chain ReactionRegenerative EndodonticsAnalysis of VarianceCell MovementCells, CulturedDental PapillaFeasibility StudiesHumansReference ValuesReproducibility of ResultsIntercellular Signaling Peptides and Proteins

Identifiers

PMID38896639
PMCPMC11178352

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.