Evidence map›Paper›PMID 38896324›Full record

ArticleArchives of microbiology2024

The role of NtrC in the adaptation of Herbaspirillum seropedicae SmR1 to nitrogen limitation and to nitrate.

Paloma Bonato, Doumit Camilios-Neto, Michelle Zibetti Tadra-Sfeir, Francisco J Teles Mota, Marcelo Muller-Santos, Roseli Wassem, Emanuel Maltempi de Souza, Fábio de Oliveira Pedrosa, Leda Satie Chubatsu

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Article in Archives of microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Paloma BonatoDepartment of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Doumit Camilios-NetoDepartment of Biochemistry and Biotechnology, Universidade Estadual de Londrina, Londrina, PR, Brazil.
Michelle Zibetti Tadra-SfeirDepartment of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Francisco J Teles MotaDepartment of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Marcelo Muller-SantosDepartment of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Roseli WassemDepartment of Genetics, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Emanuel Maltempi de SouzaDepartment of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Fábio de Oliveira PedrosaDepartment of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Leda Satie ChubatsuDepartment of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil. chubatsu@ufpr.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The RNA-Seq profiling of Herbaspirillum seropedicae SmR1 wild-type and ntrC mutant was performed under aerobic and three nitrogen conditions (ammonium limitation, ammonium shock, and nitrate shock) to identify the major metabolic pathways modulated by these nitrogen sources and those dependent on NtrC. Under ammonium limitation, H. seropedicae scavenges nitrogen compounds by activating transporter systems and metabolic pathways to utilize different nitrogen sources and by increasing proteolysis, along with genes involved in carbon storage, cell protection, and redox balance, while downregulating those involved in energy metabolism and protein synthesis. Growth on nitrate depends on the narKnirBDHsero_2899nasA operon responding to nitrate and NtrC. Ammonium shock resulted in a higher number of genes differently expressed when compared to nitrate. Our results showed that NtrC activates a network of transcriptional regulators to prepare the cell for nitrogen starvation, and also synchronizes nitrogen metabolism with carbon and redox balance pathways.

Indexed as

Bacterial ProteinsGene Expression Regulation, BacterialHerbaspirillumNitratesNitrogenAdaptation, PhysiologicalAmmonium CompoundsCarbonMetabolic Networks and PathwaysAmmonium CompoundsBacterial ProteinsCarbonNitratesNitrogenAssimilatory Nitrate ReductaseHerbaspirillum seropedicaeNitrate metabolismNitrogen metabolismNtrC regulon

Identifiers

PMID38896324

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.