ArticleHorticulture research2024
An efficient protoplast-based genome editing protocol for
Article in Horticulture research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Multifunctional Vitamin BAntioxidants (Basel, Switzerland) · 2026Article
- Optimized protocol for efficient generation, confirmation, transformation, and CRISPR editing of grapevine hairy roots.Journal of experimental botany · 2026Article
- A high-throughput heterologous expression platform for plant synthetic biology based on Arabidopsis suspension cells.Journal of experimental botany · 2026Article
- Haplotype graph analysis of PdR1 uncovers resistance diversity to Pierce's disease in Vitis arizonica and its hybrids.G3 (Bethesda, Md.) · 2026Article
- Optimization of CRISPR/Cas9-mediated CtPDS knockout in guar protoplasts.The plant genome · 2026Article
- Induction of Embryogenic Callus, Protoplast Isolation, and PEG-Mediated Transformation Protocols inPlants (Basel, Switzerland) · 2026Article
- Biodiversity and genetic improvement ofFrontiers in plant science · 2026Review
- Transforming viticulture through genomic innovation and integrated pest management for sustainable grape production.NPJ science of plants · 2026Review
- CRISPR/Cas9-based gene-editing platform development by targeting genes encoding magnesium chelatase and phytoene desaturase in sugarbeet (Frontiers in genome editing · 2026Article
- A novel application of Raman spectroscopy to monitor cell wall removal during protoplasting in grapevine.Frontiers in plant science · 2026Article
- Establishing a reliable protoplast system for grapevine: isolation, transformation, and callus induction.Protoplasma · 2025Article
- In Vitro Regeneration of Southern Italian Grapevine Cultivars from Embryogenic Calluses and Protoplasts.Plants (Basel, Switzerland) · 2025Article
- Advances in grape and pathogen genomics toward durable grapevine disease resistance.Journal of experimental botany · 2025Review
- Protoplast-Based Regeneration Enables CRISPR/Cas9 Application in Two TemperatePlants (Basel, Switzerland) · 2025Article
- Streamlined protoplast transfection system for in-vivo validation and transgene-free genome editing in Banana.Transgenic research · 2025Article
- CRISPR/Cas9-driven double modification of grapevine MLO6-7 imparts powdery mildew resistance, while editing of NPR3 augments powdery and downy mildew tolerance.The Plant journal : for cell and molecular biology · 2025Article
- Advances in the molecular mechanism of grapevine resistance to fungal diseases.Molecular horticulture · 2025Review
- DNA-free CRISPR genome editing in raspberry (Frontiers in genome editing · 2025Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR-Cas technologies allow for precise modifications in plant genomes and promise to revolutionize agriculture. These technologies depend on the delivery of editing components into plant cells and the regeneration of fully edited plants. In vegetatively propagated plants, such as grape, protoplast culture provides one of the best avenues for producing non-chimeric and transgene-free genome-edited plants. However, poor regeneration of plants from protoplasts has hindered their implementation for genome editing. Here, we report an efficient protocol for regenerating plants from protoplasts from multiple grape varieties. By encapsulating the protoplasts in calcium alginate beads and co-culturing them with feeder cultures, the protoplasts divide to form callus colonies that regenerate into embryos and ultimately plants. This protocol worked successfully in wine and table grape (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.