Tracking and recording of intracellular oxygen concentration changes in cell organelles: preparation and function of azide-modified fluorescent probes.

Hiroki Makanai, Miei Kanda, Sae Harada, Tatsuya Nishihara, Kazuhito Tanabe

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Article in RSC advances, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Hiroki MakanaiDepartment of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University 5-10-1 Fuchinobe, Chuo-ku Sagamihara 252-5258 Japan tanabe.kazuhito@chem.aoyama.ac.jp.

Miei KandaDepartment of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University 5-10-1 Fuchinobe, Chuo-ku Sagamihara 252-5258 Japan tanabe.kazuhito@chem.aoyama.ac.jp.

Sae HaradaDepartment of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University 5-10-1 Fuchinobe, Chuo-ku Sagamihara 252-5258 Japan tanabe.kazuhito@chem.aoyama.ac.jp.

Tatsuya NishiharaDepartment of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University 5-10-1 Fuchinobe, Chuo-ku Sagamihara 252-5258 Japan tanabe.kazuhito@chem.aoyama.ac.jp.ORCID https://orcid.org/0000-0002-6020-8757

Kazuhito TanabeDepartment of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University 5-10-1 Fuchinobe, Chuo-ku Sagamihara 252-5258 Japan tanabe.kazuhito@chem.aoyama.ac.jp.ORCID https://orcid.org/0000-0003-2440-2293

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tracking hypoxic environments and changes in oxygen levels contribute to the elucidation of pathological mechanisms. In this study, we attempted to design molecular probes that can be activated to show fluorescence under hypoxic conditions and that can move to specific cell organelles. Considering that azide groups were selectively reduced to primary amines by reductases under hypoxic conditions, we prepared Hoechst and fluorophore Cy-5 derivatives with azide groups (Hoechst-N

Identifiers

PMID38895527

DOI10.1039/d4ra01625d

PMCPMC11184654

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.