Enrichment of Trypsin Inhibitor from Soybean Whey Wastewater Using Different Precipitating Agents and Analysis of Their Properties.

Yongsheng Zhou, Siyun Zhou, Cuiwen Lu, Yihao Zhang, Haiyan Zhao

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Yongsheng ZhouCollege of Food and Biochemical Engineering, Guangxi Science & Technology Normal University, Laibin 546199, China.

Siyun ZhouCollege of Food and Biochemical Engineering, Guangxi Science & Technology Normal University, Laibin 546199, China.

Cuiwen LuCollege of Food and Biochemical Engineering, Guangxi Science & Technology Normal University, Laibin 546199, China.

Yihao ZhangCollege of Food and Biochemical Engineering, Guangxi Science & Technology Normal University, Laibin 546199, China.

Haiyan ZhaoCollege of Food and Biochemical Engineering, Guangxi Science & Technology Normal University, Laibin 546199, China.

Funding

the Basic Ability Enhancement Program for Young and Middle-aged Teachers in Guangxi Colleges and Universities 2024KY0864

6 · The paper itself

Abstract

Recovering valuable active substances from the by-products of agricultural processing is a crucial concern for scientific researchers. This paper focuses on the enrichment of soybean trypsin inhibitor (STI) from soybean whey wastewater using either ammonium sulfate salting or ethanol precipitation, and discusses their physicochemical properties. The results show that at a 60% ethanol content, the yield of STI was 3.983 mg/mL, whereas the yield was 3.833 mg/mL at 60% ammonium sulfate saturation. The inhibitory activity of STI obtained by ammonium sulfate salting out (A-STI) was higher than that obtained by ethanol precipitation (E-STI). A-STI exhibited better solubility than E-STI at specific temperatures and pH levels, as confirmed by turbidity and surface hydrophobicity measurements. Thermal characterization revealed that both A-STI and E-STI showed thermal transition temperatures above 90 °C. Scanning electron microscopy demonstrated that A-STI had a smooth surface with fewer pores, while E-STI had a rough surface with more pores. In conclusion, there was no significant difference in the yield of A-STI and E-STI (

Indexed as

Glycine maxTrypsin InhibitorsWastewaterWheyAmmonium SulfateChemical PrecipitationHydrogen-Ion ConcentrationHydrophobic and Hydrophilic InteractionsSolubilityTemperatureAmmonium SulfateTrypsin InhibitorsWastewaterethanol precipitationsoybean wheytrypsin inhibitor

Identifiers

PMID38893489

DOI10.3390/molecules29112613

PMCPMC11173672

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