ArticleInternational journal of molecular sciences2024
Bowman-Birk Inhibitor Mutants of Soybean Generated by CRISPR-Cas9 Reveal Drastic Reductions in Trypsin and Chymotrypsin Inhibitor Activities.
Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Targeted Reduction of Specific Antinutritional and Allergen-Associated Proteins to Advance Soybean Seed Quality.International journal of molecular sciences · 2026Article
- Multiplex gene editing drives revolution in crop breeding: overlaid editing of multiple genes and customization of complex traits.Advanced biotechnology · 2026Review
- Climate change and the antinutrient-antioxidant puzzle in common bean seeds.Journal of the science of food and agriculture · 2026Review
- Metabolic engineering of soybean for improving grain quality for animal consumption.Frontiers in plant science · 2026Review
- CRISPR/Cas9 in soybean research: a measured leap toward genetic refinement.Frontiers in genome editing · 2026Review
- Targets and strategies to design soybean seed composition traits.The plant genome · 2025Review
- Dietary protein sources in concentrate supplementation influence growth performance by manipulating gut microbiota and serum metabolites in suckling Donkey foals.Animal microbiome · 2025Article
- Seed-Specific Silencing of Abundantly Expressed Soybean Bowman-Birk Protease Inhibitor Genes by RNAi Lowers Trypsin and Chymotrypsin Inhibitor Activities and Enhances Protein Digestibility.International journal of molecular sciences · 2025Article
- Gene expression and evolution of Bowman-Birk protease inhibitors in wild and domesticatedFrontiers in plant science · 2025Article
- Advances in Soybean Genetic Improvement.Plants (Basel, Switzerland) · 2024Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Despite the high quality of soybean protein, raw soybeans and soybean meal cannot be directly included in animal feed mixtures due to the presence of Kunitz (KTi) and Bowman-Birk protease inhibitors (BBis), which reduces animal productivity. Heat treatment can substantially inactivate trypsin and chymotrypsin inhibitors (BBis), but such treatment is energy-intensive, adds expense, and negatively impacts the quality of seed proteins. As an alternative approach, we have employed CRISPR/Cas9 gene editing to create mutations in
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Registered trials
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