Observational studyJournal of assisted reproduction and genetics2024
Optimized sperm selection: a highly efficient device for the isolation of progressive motile sperm with low DNA fragmentation index.
Observational study in Journal of assisted reproduction and genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Nanotechnology in reproductive medicine: from gamete engineering to precision therapeutics.RSC advances · 2026Review
- Intracytoplasmic Sperm Injection Clinical Outcomes: A Non-Inferiority Comparison of The Novel Felix™ Electrophoretic System and Conventional Density Gradient Centrifugation for Sperm Selection, A Prospective Clinical Trial.International journal of fertility & sterility · 2026Article
- Sperm Selection Procedures in Cases of High Sperm DNA Fragmentation Index.Advances in experimental medicine and biology · 2026Review
- Density gradient centrifugation specifically improves sperm motility in hyperuricemia: evidence from intrauterine insemination cycles - retrospective cohort study.Basic and clinical andrology · 2025Article
- Integrating Lifestyle Modifications and Advanced Sperm Separation Technology: A Case Study on Overcoming Male Infertility Using the LensHooke CA0 Device.Journal of pharmacy & bioallied sciences · 2025Article
- Sperm Separation and Selection Techniques to Mitigate Sperm DNA Damage.Life (Basel, Switzerland) · 2025Review
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Authors and funding
11 authors.
Funding
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Abstract
purposeTo identify the sperm preparation procedure that selects the best sperm population for medically assisted reproduction.
methodsProspective observational study comparing the effect of four different sperm selection procedures on various semen parameters. Unused raw semen after routine diagnostic analysis was split in four fractions and processed by four different methods: (1) density gradient centrifugation (DGC), (2) sperm wash (SW), (3) DGC followed by magnetic activated cell sorting (MACS), and (4) using a sperm separation device (SSD). Each fraction was analyzed for progressive motility, morphology, acrosome index (AI), and DNA fragmentation index (DFI).
resultsWith DGC as standard of care in intraclass correlation coefficient analysis, only SSD was in strong disagreement regarding progressive motility and DFI [0.26, 95%CI (- 0.2, 0.58), and 0.17, 95%CI (- 0.19, 0.45), respectively]. When controlling for abstinence duration, DFI was significantly lower after both MACS and SSD compared to DGC [- 0.27%, 95%CI (- 0.47, - 0.06), p = 0.01, and - 0.6%, 95%CI (- 0.80, - 0.41), p < 0.001, respectively]. Further comparisons between SSD and MACS indicate significantly less apoptotic cells [Median (IQR) 4 (5), 95%CI (4.1, - 6.8) vs Median (IQR) 5 (8), 95%CI (4.9, - 9.2), p < 0.001, respectively] and dead cells [Median (IQR) 9.5 (23.3), 95%CI (13.2, - 22.4) vs Median (IQR) 22 (28), 95%CI (23.1, - 36.8), p < 0.001, respectively] in the SSD group.
conclusionThe selection of a population of highly motile spermatozoa with less damaged DNA from unprocessed semen is ideally performed with SSD. Question remains whether this method improves the embryological outcomes in the IVF laboratory.
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