Evidence map›Paper›PMID 38888878›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2024

Kinesin motor KIF16A regulates microtubule stability and actin-dependent spindle migration in mouse oocyte meiosis.

Wei Wang, Zhenhu Shi, Dandan Zhang, Wenwen Hou, Huijie Ma, Xinyu Liu, Yongteng Zhang, Jinbao Zhu, Zaishan Yang, Bo Jia and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Kinesin motor KIF16A regulates microtubule stability and actin-dependent spindle migration in mouse oocyte meiosis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Wei WangCollege of Animal Science and Technology, Anhui Agricultural University, Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, Hefei, China.ORCID https://orcid.org/0009-0001-8541-8582
Zhenhu ShiCollege of Animal Science and Technology, Anhui Agricultural University, Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, Hefei, China.ORCID https://orcid.org/0009-0006-2109-305X
Dandan ZhangDepartment of Reproductive Medicine, General Hospital of Wanbei Coal Group, Key Laboratory of Reproductive Medicine and Embryo of Suzhou City, Suzhou, China.ORCID https://orcid.org/0000-0001-9011-2660
Wenwen HouCenter of Reproductive Medicine, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, China.ORCID https://orcid.org/0000-0002-4903-5795
Huijie MaCollege of Animal Science and Technology, Anhui Agricultural University, Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, Hefei, China.ORCID https://orcid.org/0009-0007-3610-0897
Xinyu LiuCollege of Animal Science and Technology, Anhui Agricultural University, Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, Hefei, China.ORCID https://orcid.org/0009-0009-9294-4060
Yongteng ZhangCollege of Animal Science and Technology, Anhui Agricultural University, Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, Hefei, China.ORCID https://orcid.org/0009-0001-3254-7575
Jinbao ZhuCollege of Animal Science and Technology, Anhui Agricultural University, Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, Hefei, China.ORCID https://orcid.org/0009-0001-6244-2547
Zaishan YangCollege of Animal Science and Technology, Anhui Agricultural University, Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, Hefei, China.ORCID https://orcid.org/0009-0003-6350-7132
Bo JiaCollege of Animal Science and Technology, Anhui Agricultural University, Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, Hefei, China.ORCID https://orcid.org/0009-0004-4096-3343
Qimei XuCollege of Animal Science and Technology, Anhui Agricultural University, Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, Hefei, China.ORCID https://orcid.org/0009-0000-8932-8974
Yunhai ZhangCollege of Animal Science and Technology, Anhui Agricultural University, Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, Hefei, China.ORCID https://orcid.org/0000-0001-9721-5910
Mianqun ZhangCollege of Animal Science and Technology, Anhui Agricultural University, Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, Hefei, China.ORCID https://orcid.org/0000-0001-5110-174X

Funding

Anhui Agricultural University (AAU) RC392004| Anhui Provincial Quality Engineering Project () 2308085QC82MOST | NSFC | National Natural Science Foundation of China-China Academy of General Technology Joint Fund for Basic Research (NSFC-) 32272881| Natural Science Foundation of Anhui Province () KJ2020A0123
6 · The paper itself

Abstract

Kif16A, a member of the kinesin-3 family of motor proteins, has been shown to play crucial roles in inducing mitotic arrest, apoptosis, and mitotic cell death. However, its roles during oocyte meiotic maturation have not been fully defined. In this study, we report that Kif16A exhibits unique accumulation on the spindle apparatus and colocalizes with microtubule fibers during mouse oocyte meiotic maturation. Targeted depletion of Kif16A using gene-targeting siRNA disrupts the progression of the meiotic cell cycle. Furthermore, Kif16A depletion leads to aberrant spindle assembly and chromosome misalignment in oocytes. Our findings also indicate that Kif16A depletion reduces tubulin acetylation levels and compromises microtubule resistance to depolymerizing drugs, suggesting its crucial role in microtubule stability maintenance. Notably, we find that the depletion of Kif16A results in a notably elevated incidence of defective kinetochore-microtubule attachments and the absence of BubR1 localization at kinetochores, suggesting a critical role for Kif16A in the activation of the spindle assembly checkpoint (SAC) activity. Additionally, we observe that Kif16A is indispensable for proper actin filament distribution, thereby impacting spindle migration. In summary, our findings demonstrate that Kif16A plays a pivotal role in regulating microtubule and actin dynamics crucial for ensuring both spindle assembly and migration during mouse oocyte meiotic maturation.

Indexed as

KinesinsMeiosisMicrotubulesOocytesSpindle ApparatusActinsAnimalsFemaleKinetochoresMiceActinsKinesinsKIF16Akinetochore‐microtubule attachmentoocyte meiosisspindle assemblyspindle migration

Identifiers

PMID38888878
PMCPMC13688060

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.