Evidence map›Paper›PMID 38888786›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Evaluating Cationic Nanoparticles as Carriers of Antiviral Nucleic Acids.

Nicholas Jadaa, Natalie Aldor, Kristof Jenik, Shayne Oberhoffner, Carly Butkowsky, Andy Tran, Aaron Lewis, Stephanie J DeWitte-Orr, Sarah Poynter

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Nicholas JadaaWilfrid Laurier University, Waterloo, ON, Canada.
Natalie AldorWilfrid Laurier University, Waterloo, ON, Canada.
Kristof JenikWilfrid Laurier University, Waterloo, ON, Canada.
Shayne OberhoffnerWilfrid Laurier University, Waterloo, ON, Canada.
Carly ButkowskyWilfrid Laurier University, Waterloo, ON, Canada.
Andy TranWilfrid Laurier University, Waterloo, ON, Canada.
Aaron LewisWilfrid Laurier University, Waterloo, ON, Canada.
Stephanie J DeWitte-OrrWilfrid Laurier University, Waterloo, ON, Canada.
Sarah PoynterWilfrid Laurier University, Waterloo, ON, Canada. spoynter@wlu.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nanoparticle carriers enable the multivalent delivery of nucleic acids to cells and protect them from degradation. In this chapter, we present a comprehensive overview of four methodologies: electrophoretic mobility shift assay (EMSA), alamarBlue/CFDA-AM cell viability dyes, fluorescence microscopy, and antiviral assays, which collectively are tools to explore interactions between nucleic acids and nanoparticles, and their biological efficacy. These assays provide insights into binding potential, cytotoxicity, and antiviral efficacy of nucleic acid-based nanoparticle treatments furthering the development of effective antiviral therapeutics.

Indexed as

NanoparticlesNucleic AcidsAnimalsCationsCell SurvivalDrug CarriersElectrophoretic Mobility Shift AssayHumansMicroscopy, FluorescenceCationsDrug CarriersNucleic AcidsAntiviralAntiviral immunityCell viabilitydsRNAElectrophoretic mobility shift assayImmunocytochemistryInnate immunityNanoparticlesNucleic acid deliveryPathogen-associated molecular pattern

Identifiers

PMID38888786

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.