Evidence map›Paper›PMID 38888781›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Bacteriophages Isolation and Efficacy Testing.

Ayesha Lone, Carlos E Martinez-Soto, Hany Anany

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Pharmacology of Bacteriophage Therapy in Children: A Re-Emerging Paradigm in Antibacterial Therapy.The journal of pediatric pharmacology and therapeutics : JPPT : the official journal of PPAG · 2026
    Article
  2. Genomic and functional characterization ofFrontiers in microbiology · 2026
    Article
  3. Phage-Based Control ofViruses · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ayesha LoneGuelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
Carlos E Martinez-SotoGuelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
Hany AnanyGuelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada. hany.anany@agr.gc.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacteriophages (phages) are viruses that infect bacteria and are the most abundant biological entity on the planet. Phages have gained popularity as an alternative to antibiotics due to their specificity and ability to efficiently lyse antimicrobial resistant bacterial pathogens. Before using phages, they must be isolated from the environment and tested to ensure purity and lytic ability against various hosts. This protocol walks through the entire multi-day procedure of enriching and processing raw environmental samples (seawater, primary sludge, and soil), testing for lytic activity, selecting and picking potential phage plaques, verifying phage purity, and finally, propagation (liquid and solid) of phages to obtain high-titer crude phage lysates.

Indexed as

BacteriophagesBacteriaSewageSoil MicrobiologySewageBacteriophageDouble-layer agar methodsIsolationLytic phageLytic plaquesPropagationPurification

Identifiers

PMID38888781

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.