Evidence map›Paper›PMID 38888776›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Establishing Air-Liquid Interface (ALI) Airway Culture Models for Infectious Disease Research.

Kim R Chiok, Nuraina A Dahlan, Arinjay Banerjee, Neeraj Dhar

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kim R ChiokVaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada.
Nuraina A DahlanVaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada.
Arinjay BanerjeeVaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada.
Neeraj DharVaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada. neeraj.dhar@usask.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Air-liquid interface (ALI) airway culture models serve as a powerful tool to emulate the characteristic features of the respiratory tract in vitro. These models are particularly valuable for studying emerging respiratory viral and bacterial infections. Here, we describe an optimized protocol to obtain the ALI airway culture models using normal human bronchial epithelial cells (NHBECs). The protocol outlined below enables the generation of differentiated mucociliary airway epithelial cultures by day 28 following exposure to air.

Indexed as

Cell Culture TechniquesEpithelial CellsAirBronchiCells, CulturedCommunicable DiseasesHumansRespiratory MucosaAir-liquid interface (ALI) airway cultureHost-pathogen interactionIn vitro models

Identifiers

PMID38888776

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.