Evidence map›Paper›PMID 38888725›Full record

ArticleMarine biotechnology (New York, N.Y.)2024

Accumulation and Phagocytosis of Fluorescently Visualized Macrophages Against Edwardsiella piscicida Infection in Established mpeg1.1-Transgenic Japanese Medaka Oryzias latipes.

Juna Yamamoto, Hana Deguchi, Takechiyo Sumiyoshi, Kentaro Nakagami, Akatsuki Saito, Hiroshi Miyanishi, Masakazu Kondo, Tomoya Kono, Masahiro Sakai, Masato Kinoshita and 1 more

Abstract read
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In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Juna YamamotoCourse of Biochemistry and Applied Biosciences, Graduate School of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.
Hana DeguchiCourse of Biochemistry and Applied Biosciences, Graduate School of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.
Takechiyo SumiyoshiCourse of Biochemistry and Applied Biosciences, Graduate School of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.
Kentaro NakagamiCourse of Biochemistry and Applied Biosciences, Graduate School of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.
Akatsuki SaitoDepartment of Veterinary Medicine, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.
Hiroshi MiyanishiDepartment of Marine Biology and Environmental Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.
Masakazu KondoDepartment of Applied Aquabiology, National Fisheries University, Japan Fisheries Research and Education Agency, Yamaguchi, 759-6595, Japan.
Tomoya KonoCourse of Biochemistry and Applied Biosciences, Graduate School of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.
Masahiro SakaiCourse of Biochemistry and Applied Biosciences, Graduate School of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.
Masato KinoshitaDivision of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
Jun-Ichi HikimaCourse of Biochemistry and Applied Biosciences, Graduate School of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan. jhikima@cc.miyazaki-u.ac.jp.ORCID http://orcid.org/0000-0002-4184-7422

Funding

Japan Agency for Medical Research and Development JP21wm0325050Japan Society for the Promotion of Science 22H02423
6 · The paper itself

Abstract

Intracellular bacteria such as those belonging to the genus Edwardsiella can survive and proliferate within macrophages. However, the detailed mechanisms underlying the host macrophage immune response and pathogen evasion strategies remain unknown. To advance the field of host macrophage research, we successfully established transgenic (Tg) Japanese medaka Oryzias latipes that possesses fluorescently visualized macrophages. As a macrophage marker, the macrophage-expressed gene 1.1 (mpeg1.1) was selected because of its predominant expression across various tissues in medaka. To validate the macrophage characteristics of the fluorescently labeled cells, May-Grünwald Giemsa staining and peroxidase staining were conducted. The labeled cells exhibited morphological features consistent with those of monocyte/macrophage-like cells and tested negative for peroxidase activity. Through co-localization studies, the fluorescently labeled cells co-localized with E. piscicida in the intestines and kidneys of infected medaka larvae, confirming the ingestion of bacteria through phagocytosis. In addition, the labeled cells expressed macrophage markers but lacked a neutrophil marker. These results suggested that the fluorescently labeled cells of Tg[mpeg1.1:mCherry/mAG] medaka were monocytes/macrophages, which will be useful for future studies aimed at understanding the mechanisms of macrophage-mediated bacterial infections.

Indexed as

Animals, Genetically ModifiedEdwardsiellaEnterobacteriaceae InfectionsFish DiseasesMacrophagesOryziasPhagocytosisAnimalsLarva2A peptideEdwardsiella piscicidaJapanese medaka Oryzias latipesMacrophage markerMPEG1

Identifiers

PMID38888725

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.