ArticleThe EMBO journal2024
Latrophilin-2 mediates fluid shear stress mechanotransduction at endothelial junctions.
Article in The EMBO journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Loss of Smad4 drives vascular malformations via c-KIT-dependent high-fluid shear stress mimicry.The Journal of clinical investigation · 2026Article
- Review
- Pulsatile flow dynamics maintain pulmonary artery architecture.JCI insight · 2026Article
- Adhesion G protein-coupled receptors.Pharmacological reviews · 2026Review
- Endothelial ADGRF5(GPR116) governs vascular adaptation required for sustained thermogenic remodeling of brown adipose tissue.Molecular metabolism · 2026Article
- EndothelialThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Retinal arteriovenous crossings as spatiotemporal stress fields: a quantitative Doppler OCT flowmetry study.Scientific reports · 2026Article
- Nanomaterials and exercise interventions: A synergistic approach for atherosclerosis therapy (Review).International journal of molecular medicine · 2026Review
- Vascular Mechanobiology: From Membrane to Nucleus.Advances in experimental medicine and biology · 2026Review
- Multidimensional optimization of stent design for endothelial shear stress regulation: Geometric structuring, surface functionalization strategies to mitigate thrombosis and restenosis.International journal of cardiology. Heart & vasculature · 2025Review
- In Vivo Models of Cardiovascular Disease:Biomedicines · 2025Review
- Direct Activity Measurement of Heterotrimeric Gi Proteins and Gq Protein By Effector Pulldown.Bio-protocol · 2025Article
- Deciphering complexity of GPCR signaling and modulation: implications and perspectives for drug discovery.Clinical science (London, England : 1979) · 2025Review
- Review
- Tie-2 regulates endothelial morphological responses to shear stress by FOXO1-triggered autophagy.PloS one · 2025Article
- VE-PTP controls a fluid shear stress set point that governs cell morphological responses through Tie-2.Frontiers in cell and developmental biology · 2025Article
- Article
- Controversy in mechanotransduction - the role of endothelial cell-cell junctions in fluid shear stress sensing.Journal of cell science · 2024Article
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14 authors.
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Abstract
Endothelial cell responses to fluid shear stress from blood flow are crucial for vascular development, function, and disease. A complex of PECAM-1, VE-cadherin, VEGF receptors (VEGFRs), and Plexin D1 located at cell-cell junctions mediates many of these events. However, available evidence suggests that another mechanosensor upstream of PECAM-1 initiates signaling. Hypothesizing that GPCR and Gα proteins may serve this role, we performed siRNA screening of Gα subunits and found that Gαi2 and Gαq/11 are required for activation of the junctional complex. We then developed a new activation assay, which showed that these G proteins are activated by flow. We next mapped the Gα residues required for activation and developed an affinity purification method that used this information to identify latrophilin-2 (Lphn2/ADGRL2) as the upstream GPCR. Latrophilin-2 is required for all PECAM-1 downstream events tested. In both mice and zebrafish, latrophilin-2 is required for flow-dependent angiogenesis and artery remodeling. Furthermore, endothelial-specific knockout demonstrates that latrophilin plays a role in flow-dependent artery remodeling. Human genetic data reveal a correlation between the latrophilin-2-encoding Adgrl2 gene and cardiovascular disease. Together, these results define a pathway that connects latrophilin-dependent G protein activation to subsequent endothelial signaling, vascular physiology, and disease.
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