ArticleNature plants2024
Overriding Mendelian inheritance in Arabidopsis with a CRISPR toxin-antidote gene drive that impairs pollen germination.
Article in Nature plants, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Hybrid origin and phenotype evolution of the modern maize.Journal of integrative plant biology · 2026Review
- From gametocides to gene drive: natural transmission distortion systems as tools for cereal breeding.Journal of applied genetics · 2026Review
- Seed dormancy shapes gene drive dynamics in plants.Nature plants · 2026Article
- A distorter-restorer system drives quantitative reproductive isolation in rice.Nature plants · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Strategies to improve the efficiency of homing gene drives with multiplexed gRNAs.BMC biology · 2025Article
- Predicting the invasiveness of threshold-dependent gene drives.bioRxiv : the preprint server for biology · 2025Article
- Effect of high temperature on pollen grains and yield in economically important crops: a review.Planta · 2025Review
- Applications and status of gene drive in plants.PLoS biology · 2025Article
- A Comparative Assessment of Self-limiting Genetic Control Strategies for Population Suppression.Molecular biology and evolution · 2025Article
- Gene drive-based population suppression in the malaria vector Anopheles stephensi.Nature communications · 2025Article
- Identification of novel genes responsible for a pollen killer present in local natural populations of Arabidopsis thaliana.PLoS genetics · 2025Article
- Applications of CRISPR Technologies in Forestry and Molecular Wood Biotechnology.International journal of molecular sciences · 2024Review
- Altering traits and fates of wild populations with Mendelian DNA sequence modifying Allele Sails.Nature communications · 2024Article
- Cleave and Rescue gamete killers create conditions for gene drive in plants.bioRxiv : the preprint server for biology · 2024Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Synthetic gene drives, inspired by natural selfish genetic elements and transmitted to progeny at super-Mendelian (>50%) frequencies, present transformative potential for disseminating traits that benefit humans throughout wild populations, even facing potential fitness costs. Here we constructed a gene drive system in plants called CRISPR-Assisted Inheritance utilizing NPG1 (CAIN), which uses a toxin-antidote mechanism in the male germline to override Mendelian inheritance. Specifically, a guide RNA-Cas9 cassette targets the essential No Pollen Germination 1 (NPG1) gene, serving as the toxin to block pollen germination. A recoded, CRISPR-resistant copy of NPG1 serves as the antidote, providing rescue only in pollen cells that carry the drive. To limit potential consequences of inadvertent release, we used self-pollinating Arabidopsis thaliana as a model. The drive demonstrated a robust 88-99% transmission rate over two successive generations, producing minimal resistance alleles that are unlikely to inhibit drive spread. Our study provides a strong basis for rapid genetic modification or suppression of outcrossing plant populations.
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