Evidence map›Paper›PMID 38885650›Full record

ArticleCell2024

A metabolomics pipeline highlights microbial metabolism in bloodstream infections.

Jared R Mayers, Jack Varon, Ruixuan R Zhou, Martin Daniel-Ivad, Courtney Beaulieu, Amrisha Bhosle, Nathaniel R Glasser, Franziska M Lichtenauer, Julie Ng, Mayra Pinilla Vera and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Polyamine Metabolism in Brain Health and Disease.Neuropharmacology and therapy · 2026
    Article
  12. Review
  13. Review
  14. Article
  15. Article
  16. Metabolomic profiling ofmSystems · 2025
    Article
  17. Article
  18. Article
  19. Article
  20. Dissecting inflammation in the immunemetabolomic era.Cellular and molecular life sciences : CMLS · 2025
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Jared R MayersDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Jack VaronDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA.
Ruixuan R ZhouDepartment of Statistics, University of Illinois at Urbana Champaign, Champaign, IL 61820, USA.
Martin Daniel-IvadDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Courtney BeaulieuBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Amrisha BhosleBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA.
Nathaniel R GlasserDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Franziska M LichtenauerDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Julie NgDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA.
Mayra Pinilla VeraDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA.
Curtis HuttenhowerBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
Mark A PerrellaDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA.
Clary B ClishBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Sihai D ZhaoDepartment of Statistics, University of Illinois at Urbana Champaign, Champaign, IL 61820, USA; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana Champaign, Champaign, IL 61820, USA.
Rebecca M BaronDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA. Electronic address: rbaron@bwh.harvard.edu.
Emily P BalskusDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA; Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA. Electronic address: balskus@chemistry.harvard.edu.

Funding

ROLE OF DIETARY CONSTITUENTS ON GENE EXPRESSION IN INTESTINAL EPITHELIUMP30DK040561 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI CHRISTOPHER P DUGGAN · 1994 to 2026
$31.6M
RESPIRATORY RESEARCHT32HL007633 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI George R Washko · 1985 to 2026
$14.9M
Therapeutic modulation of zinc for lung injury and mechanobiologyR01HL142093 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI BARON, REBECCA M, TSCHUMPERLIN, DANIEL J. · 2019 to 2022
$2.8M
Mesenchymal Stromal Cells, Autophagy, and the Host Response to Systemic Bacterial InfectionR01GM136804 · NIGMS · BRIGHAM AND WOMEN'S HOSPITAL · PI PERRELLA, MARK A · 2020 to 2023
$1.4M
NHLBI NIH HHS R01 HL142093NHLBI NIH HHS T32 HL007633NIDDK NIH HHS P30 DK040561NIGMS NIH HHS R01 GM136804
6 · The paper itself

Abstract

The growth of antimicrobial resistance (AMR) highlights an urgent need to identify bacterial pathogenic functions that may be targets for clinical intervention. Although severe infections profoundly alter host metabolism, prior studies have largely ignored microbial metabolism in this context. Here, we describe an iterative, comparative metabolomics pipeline to uncover microbial metabolic features in the complex setting of a host and apply it to investigate gram-negative bloodstream infection (BSI) in patients. We find elevated levels of bacterially derived acetylated polyamines during BSI and discover the enzyme responsible for their production (SpeG). Blocking SpeG activity reduces bacterial proliferation and slows pathogenesis. Reduction of SpeG activity also enhances bacterial membrane permeability and increases intracellular antibiotic accumulation, allowing us to overcome AMR in culture and in vivo. This study highlights how tools to study pathogen metabolism in the natural context of infection can reveal and prioritize therapeutic strategies for addressing challenging infections.

Indexed as

MetabolomicsPolyaminesAnimalsAnti-Bacterial AgentsBacteremiaFemaleGram-Negative Bacterial InfectionsHumansMiceAnti-Bacterial AgentsPolyaminesantibiotic resistancediacetylspermidinemetabolomicsN-acetylputrescinepolyamine/diamine acetyltransferasepolyaminessepsis

Identifiers

PMID38885650
PMCPMC11283678

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.