Evidence map›Paper›PMID 38884909›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Techniques for Genetic Manipulation.

Carla García López, Luis Saralegui Remón, Cristina Uruén García, Camila Bosch Díaz, Paula Jurado Romero, Marcelo Gottschalk, Jesús Arenas Busto

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Carla García LópezUnidad de Microbiología e Inmunología, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.
Luis Saralegui RemónUnidad de Microbiología e Inmunología, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.
Cristina Uruén GarcíaUnidad de Microbiología e Inmunología, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.
Camila Bosch DíazUnidad de Microbiología e Inmunología, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.
Paula Jurado RomeroUnidad de Microbiología e Inmunología, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.
Marcelo GottschalkStreptococcus suis Laboratory, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC, Canada.
Jesús Arenas BustoUnidad de Microbiología e Inmunología, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain. jaarenas@unizar.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the last few decades, molecular techniques and genetic modification have been used in genotype and phenotype studies of S. suis. Genomic modification of S. suis requires DNA acquisition and its stable insertion into the chromosome by allelic exchange. In this chapter, we described two techniques for the preparation of genomic constructs (cloning and overlapping extension PCR) and for DNA uptake (electroporation and transformation). The protocols are accompanied with examples. All described protocols were successful on our hands with the reference S. suis strain P1/7.

Indexed as

ElectroporationCloning, MolecularPolymerase Chain ReactionGenetic engineeringMutantsPlasmidStreptococcus suisTransformationTransformation

Identifiers

PMID38884909

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.