ArticleHeliyon2024
Development of a high-speed and ultrasensitive UV/Vis-CM for detecting total triterpenes in traditional Chinese medicine and its application.
Article in Heliyon, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Phyto-Assisted Synthesis of Silver Nanoparticles (Ag-NPs) Using Delonix elata Extract: Characterization, Antimicrobial, Antioxidant, Anti-Inflammatory, and Photocatalytic Activities.Molecular biotechnology · 2026Article
- Micropropagation ofPlants (Basel, Switzerland) · 2025Article
- Isolation, structural elucidation, and integrated biological and computational evaluation of antidiabetic labdane diterpenes fromRSC advances · 2025Article
- Construction and optimization of quantitative analysis models for pigments in broccoli (Food chemistry: X · 2025Article
- Prunin: An Emerging Anticancer Flavonoid.International journal of molecular sciences · 2025Review
- Deciphering nutrient stress in plants: integrative insight from metabolomics and proteomics.Functional & integrative genomics · 2025Review
- Biotransformation of tobacco-derived Z-abienol into precursors of ambrox by newly identifiedFrontiers in microbiology · 2025Article
- Cytotoxic, anti-inflammatory, antioxidant, and anti-glyoxalase-I evaluation of chelating substances: In silico and in vitro study.PloS one · 2025Article
- Therapeutic efficacy and mechanisms of gentiopicroside in various diseases.Frontiers in pharmacology · 2025Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study proposes a novel colorimetric method based on the ultraviolet/visible spectrophotometry-colorimetric method (UV/Vis-CM) for detecting and quantifying total triterpenoids in traditional Chinese medicine. By incorporating the colourants 2-hydroxy-5-methylbenzaldehyde and concentrated sulfuric acid, triterpenoid compounds colour development became more sensitive, and the detection accuracy was significantly improved. 2-hydroxy-5-methylbenzaldehyde and concentrated sulfuric acid were incorporated in a 1:3 vol ratio at room temperature to react with the total triterpenes for 25 min, incorporated to an ice bath for 5 min, and then detected at the optimal absorption wavelength. The accuracy and reliability of this method were verified by comparison with high-performance liquid chromatography and four other colorimetric methods. Additionally, this approach has the advantages of not requiring heating during operation, high sensitivity, short usage time, low solvent usage, and low equipment costs. This study not only offers a reliable method for detecting total triterpenes in traditional Chinese medicine but also offers a rapid detection tool for on-site testing and large-scale screening, laying a foundation for the modernization of traditional Chinese medicine research, quality control, and drug development.
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