ArticleOptica2023
High-throughput deep tissue two-photon microscopy at kilohertz frame rates.
Article in Optica, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed.
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- A versatile platform for two-photon neuronal population voltage imaging across cortical depths.Nature methods · 2026Article
- FACED 2.0 enables large-scale voltage and calcium imaging in vivo.Nature methods · 2026Article
- Two-photon 3D imaging of optically stimulated neural activity at 100 Hz.Light, science & applications · 2026Article
- Dual-channel event microscopy for ultrafast biological imaging.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo.bioRxiv : the preprint server for biology · 2026Article
- Dual-Channel Event Microscopy for Ultrafast Biological Imaging.bioRxiv : the preprint server for biology · 2025Article
- Optical segmentation-based compressed readout of neuronal voltage dynamics.Nature communications · 2025Article
- Non-inertial scan angle multiplier for expanded fields-of-view.bioRxiv : the preprint server for biology · 2025Article
- Ultrafast optical imaging techniques for exploring rapid neuronal dynamics.Neurophotonics · 2025Review
- Deep two-photon voltage imaging with adaptive excitation.Research square · 2024Article
- Bessel beam optical coherence microscopy enables multiscale assessment of cerebrovascular network morphology and function.Light, science & applications · 2024Article
- Review
- EventLFM: event camera integrated Fourier light field microscopy for ultrafast 3D imaging.Light, science & applications · 2024Article
- Suppression of Subpixel Jitter in Resonant Scanning Systems With Phase-locked Sampling.IEEE transactions on medical imaging · 2024Article
- High throughput detection of capillary stalling events with Bessel beam two-photon microscopy.Neurophotonics · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
High-speed laser scanning microscopes are essential for monitoring fast biological phenomena. However, existing strategies that achieve millisecond time resolution with two-photon microscopes (2PMs) are generally technically challenging and suffer from compromises among imaging field of view, excitation efficiency, and depth penetration in thick tissue. Here, we present a versatile solution that enables a conventional video-rate 2PM to perform 2D scanning at kilohertz frame rates over large fields of view. Our system is based on implementation of a scan multiplier unit that provides inertia-free multiplication of the scanning speed while preserving all the benefits of standard 2PM. We demonstrate kilohertz subcellular-resolution 2PM imaging with an order of magnitude higher imaging throughput than previously achievable and penetration depths exceeding 500 μm, which we apply to the study of neurovascular coupling dynamics in the mouse brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.