ArticleBiophysical journal2024
Impact of trypsin on cell cytoplasm during detachment of cells studied by terahertz sensing.
Article in Biophysical journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Precisely Engineering Biomimetic Nanoparticles via Controlled Cell Membrane Coating.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Reversible Sol-Gel Transition in Thermoresponsive Collagen Hydrogels for Cryogen-Free Cell Logistics.Gels (Basel, Switzerland) · 2026Article
- Adhesion-Related Macrophages Regulate Metabolic Homeostasis Through CAV-1 Dependency.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Comparative phenotypic and molecular profiling of replicative and chemically-induced senescence in articular chondrocytes.Cell death discovery · 2026Article
- Indirubin treatment was associated with modulation of the PI3K/AKT and MAPK pathways and induction of apoptosis and autophagy.Scientific reports · 2026Article
- Cocaine affects astrocyte iNOS expression.Brain research · 2026Article
- Osmotic pressure regulates DNA labelling and transcription with dCas9-SunTag system in live cells.Nature communications · 2025Article
- Polymeric Nanoparticles with Surface-Anchored Functional Groups as Chelating Agents for Calcium (CaPharmaceuticals (Basel, Switzerland) · 2025Article
- Microhand Platform Equipped with Plate-Shaped End-Effectors Enables Precise Probing of Intracellular Structure Contribution to Whole-Cell Mechanical Properties.Micromachines · 2025Article
- Integrating 3D Osteocyte Culture, Microgravity Simulation, and Fluid Flow Reveals Mechanisms of Osteocyte Mechanosensation and Calcium Signaling Altered by Disuse.Biomolecules · 2025Article
- Article
- Targeting Bacterial Biofilms on Medical Implants: Current and Emerging Approaches.Antibiotics (Basel, Switzerland) · 2025Review
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trypsin is a very common enzyme used in cell culture to harvest cells by cleaving the proteins responsible for cell adhesion. However, trypsin also induces undesirable effects on cells, such as altering membrane proteins and the cytoskeleton, changing the composition of the cytoplasm and the cell volume, and even leading to cell death when used improperly. Using attenuated total reflection in the terahertz domain, confocal microscopy, and the propidium iodide test, we quantified in real time the change in cytoplasmic content induced by trypsin proteolysis on Madin-Darby canine kidney epithelial cells. We have observed a cytoplasmic modification from the very first seconds of trypsinization, following the change of cell volume due to mechanical re-equilibrium of the membrane. We found that the cytoplasmic alteration is associated with a transfer of small solutes: electrolytes and metabolites. We also found a very good nonlinear correlation between the side effects monitored by terahertz sensing and the cell height, regardless of the dependence of the cell height on trypsin concentration and exposure time.
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