Evidence map›Paper›PMID 38877289›Full record

ArticleGenes & genomics2024

Identification and expression analysis of nuclear factor Y transcription factor genes under drought, cold and Eldana infestation in sugarcane (Saccharum spp. hybrid).

Jancke le Roux, Robyn Jacob, Riëtte Fischer, Christell van der Vyver

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Article in Genes & genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Jancke le RouxInstitute for Plant Biotechnology, Department of Genetics, University of Stellenbosch, Stellenbosch, 7602, South Africa.
Robyn JacobSouth African Sugarcane Research Institute (SASRI), KwaZulu-Natal, P/Bag X02, Mount Edgecombe, Durban, 4300, South Africa.
Riëtte FischerInstitute for Plant Biotechnology, Department of Genetics, University of Stellenbosch, Stellenbosch, 7602, South Africa.
Christell van der VyverInstitute for Plant Biotechnology, Department of Genetics, University of Stellenbosch, Stellenbosch, 7602, South Africa. cvdv@sun.ac.za.ORCID http://orcid.org/0000-0001-5362-0543

Funding

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6 · The paper itself

Abstract

backgroundThe Nuclear Factor Y (NF-Y) transcription factor (TF) gene family plays a crucial role in plant development and response to stress. Limited information is available on this gene family in sugarcane.

objectivesTo identify sugarcane NF-Y genes through bioinformatic analysis and phylogenetic association and investigate the expression of these genes in response to abiotic and biotic stress.

methodsSugarcane NF-Y genes were identified using comparative genomics from functionally annotated Poaceae and Arabidopsis species. Quantitative PCR and transcriptome analysis assigned preliminary functional roles to these genes in response to water deficit, cold and African sugarcane borer (Eldana saccharina) infestation.

resultsWe identify 21 NF-Y genes in sugarcane. Phylogenetic analysis revealed three main branches representing the subunits with potential discrepancies present in the assignment of numerical names of some NF-Y putative orthologs across the different species. Gene expression analysis indicated that three genes, ShNF-YA1, A3 and B3 were upregulated and two genes, NF-YA4 and A7 were downregulated, while three genes were upregulated, ShNF-YB2, B3 and C4, in the plants exposed to water deficit and cold stress, respectively. Functional involvement of NF-Y genes in the biotic stress response were also detected where three genes, ShNF-YA6, A3 and A7 were downregulated in the early resistant (cv. N33) response to Eldana infestation whilst only ShNF-YA6 was downregulated in the susceptible (cv. N11) early response.

conclusionsOur research findings establish a foundation for investigating the function of ShNF-Ys and offer candidate genes for stress-resistant breeding and improvement in sugarcane.

Indexed as

DroughtsGene Expression Regulation, PlantPhylogenySaccharumAnimalsCCAAT-Binding FactorCold TemperatureMothsPlant ProteinsStress, PhysiologicalCCAAT-Binding Factornuclear factor YPlant ProteinsAbiotic and biotic stress responsesEldanaNF-Y transcription factorsSugarcane

Identifiers

PMID38877289
PMCPMC11329523

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.