ArticleNature communications2024
Scanless two-photon voltage imaging.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- Three-dimensional voltage imaging in live larval zebrafish brains using fully genetically encoded voltage indicator.Scientific reports · 2026Article
- Beyond Membrane Potential: Exploiting Signal Complexity in Genetically Encoded Voltage Indicators.Sensors (Basel, Switzerland) · 2026Article
- A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo.bioRxiv : the preprint server for biology · 2026Article
- Designer indicators for two-photon recording of subthreshold voltage dynamics.Nature methods · 2026Article
- Designer indicators for two-photon recording of subthreshold voltage dynamics.bioRxiv : the preprint server for biology · 2026Article
- A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo.bioRxiv : the preprint server for biology · 2026Article
- Fluorescent Labeling Methods for Brain Structure Research.Molecules (Basel, Switzerland) · 2026Review
- Voltage imaging as a window into neural computation.Neurophotonics · 2026Article
- Snapshot 3D at the speed frontier: redefining light-field microscopy for neuroimaging.PhotoniX · 2026Article
- Voltage imaging with periodic structured illumination.Biomedical optics express · 2026Article
- Sub-threshold depolarization differentially regulated by multiple ion channels plays a key role in initiation of baroreflex afferent neurotransmission evidenced from baroreceptor terminals to its cell bodies.Frontiers of medicine · 2025Article
- High-throughput synaptic connectivity mapping using in vivo two-photon holographic optogenetics and compressive sensing.Nature neuroscience · 2025Article
- Voltage Imaging with Periodic Structured Illumination.bioRxiv : the preprint server for biology · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Window into the Brain: In Vivo Multiphoton Imaging.ACS photonics · 2025Review
- AI-based hardware and software tools in microscopy to boost research in immunology and virology.Frontiers in immunology · 2025Review
- Deep two-photon voltage imaging with adaptive excitation.Research square · 2024Article
- Recent advances in light patterned optogenetic photostimulation in freely moving mice.Neurophotonics · 2024Article
- Optical constraints on two-photon voltage imaging.Neurophotonics · 2024Article
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11 authors.
Funding
Abstract
Two-photon voltage imaging has long been heralded as a transformative approach capable of answering many long-standing questions in modern neuroscience. However, exploiting its full potential requires the development of novel imaging approaches well suited to the photophysical properties of genetically encoded voltage indicators. We demonstrate that parallel excitation approaches developed for scanless two-photon photostimulation enable high-SNR two-photon voltage imaging. We use whole-cell patch-clamp electrophysiology to perform a thorough characterization of scanless two-photon voltage imaging using three parallel illumination approaches and lasers with different repetition rates and wavelengths. We demonstrate voltage recordings of high-frequency spike trains and sub-threshold depolarizations from neurons expressing the soma-targeted genetically encoded voltage indicator JEDI-2P-Kv. Using a low repetition-rate laser, we perform multi-cell recordings from up to fifteen targets simultaneously. We co-express JEDI-2P-Kv and the channelrhodopsin ChroME-ST and capitalize on their overlapping two-photon absorption spectra to simultaneously evoke and image action potentials using a single laser source. We also demonstrate in vivo scanless two-photon imaging of multiple cells simultaneously up to 250 µm deep in the barrel cortex of head-fixed, anaesthetised mice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.