Evidence map›Paper›PMID 38874819›Full record

ArticlePlant cell reports2024

Cationic lipid nanoparticle-mediated delivery of a Cas9/crRNA ribonucleoprotein complex for transgene-free editing of the citrus plant genome.

Lamiaa M Mahmoud, Manjul Dutt

Abstract read
PubMed Publisher
In one paragraph

Article in Plant cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Substantial enhancement ofHorticulture research · 2025
    Article
  8. CRISPR/Cas system-mediated transgene-free or DNA-free genome editing in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
    Review
  9. Review
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Lamiaa M MahmoudDepartment of Horticultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA.
Manjul DuttDepartment of Horticultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA. manjul@ufl.edu.ORCID http://orcid.org/0000-0002-8027-7012

Funding

National Institute of Food and Agriculture 2022-70029-38470
6 · The paper itself

Abstract

key messageA lipofectamine-mediated transfection protocol for DNA-free genome editing of citrus protoplast cells using a Cas9/gRNA ribonucleoprotein (RNP) complex resulted in the production of transgene free genome edited citrus.

Indexed as

CitrusGene EditingGenome, PlantLipidsNanoparticlesRibonucleoproteinsCationsCRISPR-Associated Protein 9CRISPR-Cas SystemsLiposomesProtoplastsRNA, Guide, CRISPR-Cas SystemsTransgenesCationsCRISPR-Associated Protein 9Lipid NanoparticlesLipidsLipofectamineLiposomesRibonucleoproteinsRNA, Guide, CRISPR-Cas SystemsAlbino phenotypeCitrusCRISPR/Cas9Genome editingMarker-freePrecision breeding

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.