Evidence map›Paper›PMID 38874818›Full record

ArticleMolecular biology reports2024

Unravelling effects of phytochemicals from buckwheat on cholesterol metabolism and lipid accumulation in HepG2 cells and its validation through gene expression analysis.

Sabreena Bhat, Younis Majeed, Gulam Nabi Yatoo, Shahnawaz Hassan, Tamana Khan, Parvaze A Sofi, Bashir Ahmed Ganai, Khalid Majid Fazili, Sajad Majeed Zargar

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Buckwheat (Journal of agricultural and food chemistry · 2026
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Sabreena BhatCORD, University of Kashmir, Hazratbal, Srinagar, 190006, Jammu & Kashmir, India.
Younis MajeedDepartment of Biotechnology, University of Kashmir, Hazratbal, Srinagar, 190006, Jammu & Kashmir, India.
Gulam Nabi YatooDepartment of Chemistry, National Institute of Technology Srinagar, Srinagar, 190006, Jammu & Kashmir, India.
Shahnawaz HassanCORD, University of Kashmir, Hazratbal, Srinagar, 190006, Jammu & Kashmir, India.
Tamana KhanProteomics Laboratory, Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar, 190025, Jammu & Kashmir, India.
Parvaze A SofiProteomics Laboratory, Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar, 190025, Jammu & Kashmir, India.
Bashir Ahmed GanaiCORD, University of Kashmir, Hazratbal, Srinagar, 190006, Jammu & Kashmir, India. bbcganai@gmail.com.
Khalid Majid FaziliDepartment of Biotechnology, University of Kashmir, Hazratbal, Srinagar, 190006, Jammu & Kashmir, India.
Sajad Majeed ZargarProteomics Laboratory, Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar, 190025, Jammu & Kashmir, India. smzargar@skuastkashmir.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe objective of this research was to elucidate the hypocholesterolemic effects of a bioactive compound extracted from buckwheat, and to delineate its influence on the regulatory mechanisms of cholesterol metabolism. The compound under investigation was identified as quercetin. MATERIAL AND

resultsIn vitro experiments conducted on HepG2 cells treated with quercetin revealed a significant reduction in intracellular cholesterol accumulation. This phenomenon was rigorously quantified by assessing the transcriptional activity of key genes involved in the biosynthesis and metabolism of cholesterol. A statistically significant reduction in the expression of HMG-CoA reductase (HMGCR) was observed, indicating a decrease in endogenous cholesterol synthesis. Conversely, an upregulation in the expression of cholesterol 7 alpha-hydroxylase (CYP7A1) was also observed, suggesting an enhanced catabolism of cholesterol to bile acids. Furthermore, the study explored the combinatory effects of quercetin and simvastatin, a clinically utilized statin, revealing a synergistic action in modulating cholesterol levels at various dosages.

conclusionsThe findings from this research provide a comprehensive insight into the mechanistic pathways through which quercetin, a phytochemical derived from buckwheat, exerts its hypocholesterolemic effects. Additionally, the observed synergistic interaction between quercetin and simvastatin opens up new avenues for the development of combined therapeutic strategies to manage hyperlipidemia.

Indexed as

CholesterolCholesterol 7-alpha-HydroxylaseFagopyrumHydroxymethylglutaryl CoA ReductasesLipid MetabolismPhytochemicalsQuercetinAnticholesteremic AgentsGene Expression ProfilingGene Expression RegulationHep G2 CellsHumansPlant ExtractsSimvastatinAnticholesteremic AgentsCholesterolCholesterol 7-alpha-HydroxylaseCYP7A1 protein, humanHMGCR protein, humanHydroxymethylglutaryl CoA ReductasesPhytochemicalsPlant ExtractsQuercetinSimvastatinBuckwheatCYP7A1HMGCRQuercetin

Identifiers

PMID38874818

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.