Evidence map›Paper›PMID 38874663›Full record

ArticleMolecular biology reports2024

Effects of a human amniotic membrane extract on ARPE-19 cells.

Matteo Lulli, Ruggero Tartaro, Laura Papucci, Lucia Magnelli, Indu Pal Kaur, Tomaso Caporossi, Stanislao Rizzo, Antonella Mannini, Fabrizio Giansanti, Nicola Schiavone

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In one paragraph

Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Matteo LulliDepartment of Experimental and Clinical Biomedical Sciences 'Mario Serio', University of Florence, Florence, Italy.
Ruggero TartaroDepartment of NEUROFARBA, Ophthalmology, University of Florence, Careggi, Florence, Italy.
Laura PapucciDepartment of Experimental and Clinical Biomedical Sciences 'Mario Serio', University of Florence, Florence, Italy.
Lucia MagnelliDepartment of Experimental and Clinical Biomedical Sciences 'Mario Serio', University of Florence, Florence, Italy.
Indu Pal KaurUGC-Centre of Advanced Study, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India.
Tomaso CaporossiVitreoretinal Surgery Unit, Isola Tiberina Gemelli Isola Hospital, Rome, Italy.
Stanislao RizzoDepartment of Ophthalmology, Catholic University of Sacred-Heart Foundation "Policlinico Universitario A. Gemelli" IRCCS, Rome, Italy.
Antonella ManniniDepartment of Experimental and Clinical Medicine - Internal Medicine Section, University of Florence, Florence, Italy. antonella.mannini@unifi.it.
Fabrizio GiansantiDepartment of NEUROFARBA, Ophthalmology, University of Florence, Careggi, Florence, Italy.
Nicola SchiavoneDepartment of Experimental and Clinical Biomedical Sciences 'Mario Serio', University of Florence, Florence, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman Amniotic Membrane (hAM) is endowed with several biological activities and might be considered an optimal tool in surgical treatment for different ophthalmic pathologies. We pioneered the surgical use of hAM to treat retinal pathologies such as macular holes, tears, and retinal detachments, and to overcome photoreceptor damage in age-related macular degeneration. Although hAM contributed to improved outcomes, the mechanisms of its effects are not yet fully understood. The characterization and explanation of the effects of hAM would allow the adoption of this new natural product in different retinal pathologies, operative contexts, and hAM formulations. At this end, we studied the properties of a hAM extract (hAME) on the ARPE-19 cells. METHODS AND

resultsA non-denaturing sonication-based technique was developed to obtain a suitable hAME. Viability, proliferation, apoptosis, oxidative stress, and epithelial-mesenchymal transition (EMT) were studied in hAME-treated ARPE-19 cells. The hAME was able to increase ARPE-19 cell viability even in the presence of oxidative stress (H

conclusionsOur results demonstrate that the hAME retains most of the properties observed in the whole tissue by others. The hAME, other than providing a manageable research tool, could represent a cost-effective and abundant drug to treat retinal pathologies in the future.

Indexed as

AmnionApoptosisCell ProliferationCell SurvivalOxidative StressRetinal Pigment EpitheliumCell LineEpithelial-Mesenchymal TransitionHumansTissue ExtractsTissue ExtractsApoptosisARPE-19EMTHuman amniotic membranePVRRetina

Identifiers

PMID38874663
PMCPMC11178654

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.