Evidence map›Paper›PMID 38874431›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2024

Gene Electrotransfer via Conductivity-Clamped Electric Field Focusing Pivots Sensori-Motor DNA Therapeutics: "A Spoonful of Sugar Helps the Medicine Go Down".

Jeremy L Pinyon, Georg von Jonquieres, Edward N Crawford, Amr Al Abed, John M Power, Matthias Klugmann, Cherylea J Browne, David M Housley, Andrew K Wise, James B Fallon and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Toward elucidating the mechanism of cochlear stimulation by infrared light.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Jeremy L PinyonTranslational Neuroscience Facility, Department of Physiology, School of Biomedical Sciences, Graduate School of Biomedical Engineering, Tyree Institute for Health Engineering (IHealthE), UNSW, Sydney, NSW, 2052, Australia.ORCID 0000-0001-6261-3927
Georg von JonquieresTranslational Neuroscience Facility, Department of Physiology, School of Biomedical Sciences, Graduate School of Biomedical Engineering, Tyree Institute for Health Engineering (IHealthE), UNSW, Sydney, NSW, 2052, Australia.ORCID 0000-0002-7423-3355
Edward N CrawfordTranslational Neuroscience Facility, Department of Physiology, School of Biomedical Sciences, Graduate School of Biomedical Engineering, Tyree Institute for Health Engineering (IHealthE), UNSW, Sydney, NSW, 2052, Australia.ORCID 0000-0002-2158-1962
Amr Al AbedTranslational Neuroscience Facility, Department of Physiology, School of Biomedical Sciences, Graduate School of Biomedical Engineering, Tyree Institute for Health Engineering (IHealthE), UNSW, Sydney, NSW, 2052, Australia.ORCID 0000-0002-5560-4771
John M PowerTranslational Neuroscience Facility, Department of Physiology, School of Biomedical Sciences, Graduate School of Biomedical Engineering, Tyree Institute for Health Engineering (IHealthE), UNSW, Sydney, NSW, 2052, Australia.ORCID 0000-0002-2478-8416
Matthias KlugmannTranslational Neuroscience Facility, Department of Physiology, School of Biomedical Sciences, Graduate School of Biomedical Engineering, Tyree Institute for Health Engineering (IHealthE), UNSW, Sydney, NSW, 2052, Australia.ORCID 0000-0003-0574-612X
Cherylea J BrowneTranslational Neuroscience Facility, Department of Physiology, School of Biomedical Sciences, Graduate School of Biomedical Engineering, Tyree Institute for Health Engineering (IHealthE), UNSW, Sydney, NSW, 2052, Australia.ORCID 0000-0001-6758-685X
David M HousleyTranslational Neuroscience Facility, Department of Physiology, School of Biomedical Sciences, Graduate School of Biomedical Engineering, Tyree Institute for Health Engineering (IHealthE), UNSW, Sydney, NSW, 2052, Australia.ORCID 0000-0002-2484-7148
Andrew K WiseBionics Institute, 384-388 Albert Street, East Melbourne, VIC, 3002, Australia.ORCID 0000-0001-9715-8784
James B FallonBionics Institute, 384-388 Albert Street, East Melbourne, VIC, 3002, Australia.ORCID 0000-0003-2686-3886
Robert K ShepherdBionics Institute, 384-388 Albert Street, East Melbourne, VIC, 3002, Australia.ORCID 0000-0002-4239-3362
John Y LinTasmanian School of Medicine, University of Tasmania, Hobart, TAS, 7001, Australia.ORCID 0000-0002-1723-4597
Catherine McMahonFaculty of Medicine and Health Sciences, The Hearing Hub, Macquarie University, Sydney, 2109, Australia.ORCID 0000-0001-7312-6593
David McAlpineFaculty of Medicine and Health Sciences, The Hearing Hub, Macquarie University, Sydney, 2109, Australia.ORCID 0000-0001-5467-6725
Catherine S BirmanFaculty of Medicine and Health Sciences, The Hearing Hub, Macquarie University, Sydney, 2109, Australia.ORCID 0000-0002-6323-773X
Waikong LaiNextSense, Royal Institute of Deaf and Blind Children, Gladesville, NSW, 2111, Australia.ORCID 0000-0001-5415-9218
Ya Lang EnkeCochlear Limited, Macquarie University, University Avenue, Macquarie Park, NSW, 2109, Australia.ORCID 0000-0002-0124-7481
Paul M CarterCochlear Limited, Macquarie University, University Avenue, Macquarie Park, NSW, 2109, Australia.ORCID 0000-0001-9286-1630
James F PatrickCochlear Limited, Macquarie University, University Avenue, Macquarie Park, NSW, 2109, Australia.
Robert D GayCochlear Limited, Macquarie University, University Avenue, Macquarie Park, NSW, 2109, Australia.ORCID 0000-0002-0774-5342
Corinne MarieCNRS, Inserm, UTCBS, Université Paris Cité, Paris, F-75006, France.ORCID 0000-0001-9619-5700
Daniel SchermanCNRS, Inserm, UTCBS, Université Paris Cité, Paris, F-75006, France.ORCID 0000-0003-1207-1298
Nigel H LovellTranslational Neuroscience Facility, Department of Physiology, School of Biomedical Sciences, Graduate School of Biomedical Engineering, Tyree Institute for Health Engineering (IHealthE), UNSW, Sydney, NSW, 2052, Australia.ORCID 0000-0003-1637-1079
Gary D HousleyTranslational Neuroscience Facility, Department of Physiology, School of Biomedical Sciences, Graduate School of Biomedical Engineering, Tyree Institute for Health Engineering (IHealthE), UNSW, Sydney, NSW, 2052, Australia.ORCID 0000-0002-8413-588X

Funding

Australian Research Council DP151014754Australian Research Council LP0992098Australian Research Council LP140101008Macquarie UniversityNational Health and Medical Research Council APP1048764National Health and Medical Research Council APP1091646National Health and Medical Research Council APP1189113National Health and Medical Research Council APP2013285NextSensePasse & Williams Foundation supplementaryUNSW Sydney
6 · The paper itself

Abstract

Viral vectors and lipofection-based gene therapies have dispersion-dependent transduction/transfection profiles that thwart precise targeting. The study describes the development of focused close-field gene electrotransfer (GET) technology, refining spatial control of gene expression. Integration of fluidics for precise delivery of "naked" plasmid deoxyribonucleic acid (DNA) in sucrose carrier within the focused electric field enables negative biasing of near-field conductivity ("conductivity-clamping"-CC), increasing the efficiency of plasma membrane molecular translocation. This enables titratable gene delivery with unprecedently low charge transfer. The clinic-ready bionics-derived CC-GET device achieved neurotrophin-encoding miniplasmid DNA delivery to the cochlea to promote auditory nerve regeneration; validated in deafened guinea pig and cat models, leading to improved central auditory tuning with bionics-based hearing. The performance of CC-GET is evaluated in the brain, an organ problematic for pulsed electric field-based plasmid DNA delivery, due to high required currents causing Joule-heating and damaging electroporation. Here CC-GET enables safe precision targeting of gene expression. In the guinea pig, reporter expression is enabled in physiologically critical brainstem regions, and in the striatum (globus pallidus region) delivery of a red-shifted channelrhodopsin and a genetically-encoded Ca

Indexed as

Electric ConductivityGenetic TherapyGene Transfer TechniquesAnimalsCatsDisease Models, AnimalDNAElectroporationGuinea PigsPlasmidsDNACNS electrotransferCNS neuromodulationnerve regenerationnonviral gene augmentation therapyprecision gene delivery

Identifiers

PMID38874431
PMCPMC11321635

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.