Evidence map›Paper›PMID 38873827›Full record

ArticleAnimal models and experimental medicine2024

Influence of ear tags on the results of body composition analysis in mice.

He Liu, Yinghua Zhang, Peng Zhang, Weiping Teng, Zhongyan Shan, Yushu Li, Dan Wang

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Article in Animal models and experimental medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

He LiuDepartment of Endocrinology and Metabolism and the Institute of Endocrinology, NHC Key Laboratory of Diagnosis and Treatment of Thyroid Diseases, The First Hospital of China Medical University, Shenyang, China.ORCID 0009-0009-9414-733X
Yinghua ZhangSchool of Public Health China Medical University, China Medical University, Shenyang, China.
Peng ZhangShenyang Food and Drug Inspection Institute, Shenyang, China.
Weiping TengDepartment of Endocrinology and Metabolism and the Institute of Endocrinology, NHC Key Laboratory of Diagnosis and Treatment of Thyroid Diseases, The First Hospital of China Medical University, Shenyang, China.
Zhongyan ShanDepartment of Endocrinology and Metabolism and the Institute of Endocrinology, NHC Key Laboratory of Diagnosis and Treatment of Thyroid Diseases, The First Hospital of China Medical University, Shenyang, China.
Yushu LiDepartment of Endocrinology and Metabolism and the Institute of Endocrinology, NHC Key Laboratory of Diagnosis and Treatment of Thyroid Diseases, The First Hospital of China Medical University, Shenyang, China.
Dan WangShenyang Food and Drug Inspection Institute, Shenyang, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe aim of this study was to investigate the influence of marking methods on the outcomes of body composition analysis and provide guidance for the selection of marking methods in mouse body composition analysis.

methodsMale C57BL/6J mice aged 6 weeks were randomly assigned for pre- and post- ear tagging measurements. The body composition of the mice was measured using a small animal body composition analyzer, which provided measurements of the mass of fat, lean, and free fluid. Then, the mass of fat, lean and free fluid to body weight ratio was gained. Further data analysis was conducted to obtain the range and coefficient of variation in body composition measurements for each mouse. The distribution of fat and lean tissue in the mice was also analyzed by comparing the fat-to-lean ratio.

results(1) The mass of all body composition components in the ear tagging group was significantly lower than that in the control group. (2) There was a significant increase in the range and coefficient of variation of body composition measurements between the ear tagging group and the control group. (3) The fat-to-lean ratio in the ear tagging group was significantly lower than that in the control group.

conclusionsEar tagging significantly lowered the results of body composition analysis in mice and higher the results of measurement error. Therefore, ear tagging should be avoided as much as possible when conducting body composition analysis experiments in mice.

Indexed as

Body CompositionMice, Inbred C57BLAdipose TissueAnimalsEarMaleMicebody composition analysisear tagginglabeling methodstainless steeltoe clipping

Identifiers

PMID38873827
PMCPMC11369023

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.