Evidence map›Paper›PMID 38872071›Full record

ArticleBiotechnology letters2024

Mutational analysis of pig tissue factor pathway inhibitor α to increase anti-coagulation activity in pig-to-human xenotransplantation.

Chang-Hee Lee, Hyeon Jeong Lee, Si-Won Park, Jiyoon Shin, Seok-Jin Kang, In-Byung Park, Hyun Kyung Kim, Taehoon Chun

Abstract read
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In one paragraph

Article in Biotechnology letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chang-Hee LeeDepartment of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.ORCID http://orcid.org/0000-0002-4424-7203
Hyeon Jeong LeeDepartment of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.ORCID http://orcid.org/0000-0003-0277-7377
Si-Won ParkDepartment of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.ORCID http://orcid.org/0000-0002-1597-9683
Jiyoon ShinDepartment of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.ORCID http://orcid.org/0000-0001-8442-6954
Seok-Jin KangDepartment of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.ORCID http://orcid.org/0000-0002-9701-8690
In-Byung ParkDepartment of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.ORCID http://orcid.org/0000-0002-8646-8709
Hyun Kyung KimDepartment of Laboratory Medicine and Cancer Research Institute, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea.ORCID http://orcid.org/0000-0003-2401-3933
Taehoon ChunDepartment of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, 02841, Republic of Korea. tchun@korea.ac.kr.ORCID http://orcid.org/0000-0002-5940-8620

Funding

Korea Health Industry Development Institute HI13C0954
6 · The paper itself

Abstract

Blood coagulation mediated by pig tissue factor (TF), which is expressed in pig tissues, causes an instant blood-mediated inflammatory reaction during pig-to-human xenotransplantation. Previously, we generated a soluble pig tissue factor pathway inhibitor α fusion immunoglobulin (TFPI-Ig) which inhibits pig TF activity more efficiently than human TFPI-Ig in human plasma. In this study, we generated several pig TFPI-Ig mutants and tested the efficacy of these mutants in preventing pig-to-human xenogeneic blood coagulation. Structurally important amino acid residues of pig TFPI-Ig were changed into different residues by site-directed mutagenesis. Subsequently, a retroviral vector encoding each cDNA of several pig TFPI-Ig mutants was cloned and transduced into CHO-K1 cells. After establishing stable cell lines expressing each of the pig TFPI-Ig mutants, soluble proteins were produced and purified for evaluating their inhibitory effects on pig TF-mediated blood coagulation in human plasma. The replacement of K

Indexed as

Blood CoagulationLipoproteinsTransplantation, HeterologousAnimalsCHO CellsCricetulusDNA Mutational AnalysisHumansMutagenesis, Site-DirectedSwineThromboplastinlipoprotein-associated coagulation inhibitorLipoproteinsThromboplastinBlood coagulationRecombinant proteinTissue factorTissue factor pathway inhibitorXenotransplantation

Identifiers

PMID38872071

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.