Evidence map›Paper›PMID 38871953›Full record

ArticleMikrochimica acta2024

Strand displacement-triggered FRET nanoprobe tracking TK1 mRNA in living cells for ratiometric fluorimetry of nucleic acid biomarker.

Fengqi Dong, Weizhen Yan, Mingsheng Qu, Xiaofei Shang, Wuqi Dong, Zhengdong Lu, Hanyuan Zhang, Wei Du, Ruilong Zhang, Zhongping Zhang and 1 more

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Fengqi Dong *School of Basic Medical Sciences, Biopharmaceutical Research Institute, Anhui Medical University, Hefei, Anhui, 230032, China.
Weizhen Yan *The First School of Clinical Medicine, Anhui Medical University, Hefei, 230032, Anhui, China.
Mingsheng QuSchool of Basic Medical Sciences, Biopharmaceutical Research Institute, Anhui Medical University, Hefei, Anhui, 230032, China.
Xiaofei ShangSchool of Basic Medical Sciences, Biopharmaceutical Research Institute, Anhui Medical University, Hefei, Anhui, 230032, China.
Wuqi DongSchool of Basic Medical Sciences, Biopharmaceutical Research Institute, Anhui Medical University, Hefei, Anhui, 230032, China.
Zhengdong LuThe First School of Clinical Medicine, Anhui Medical University, Hefei, 230032, Anhui, China.
Hanyuan ZhangDepartment of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, China. doczhy@vip.163.com.
Wei DuSchool of Basic Medical Sciences, Biopharmaceutical Research Institute, Anhui Medical University, Hefei, Anhui, 230032, China. iamwdu@ahmu.edu.cn.
Ruilong ZhangSchool of Chemistry and Chemical Engineering, Anhui University, Hefei, 230601, Anhui, China.
Zhongping ZhangSchool of Chemistry and Chemical Engineering, Anhui University, Hefei, 230601, Anhui, China.
Tingting ZhaoSchool of Basic Medical Sciences, Biopharmaceutical Research Institute, Anhui Medical University, Hefei, Anhui, 230032, China. ttzhao@ahmu.edu.cn.ORCID http://orcid.org/0000-0001-9376-6715

Funding

Anhui Province Science Fund for Outstanding Young Scholars 2208085Y27Excellent Young Talents Fund Program of Higher Education Institutions of Anhui Province gxyqZD2021102National Natural Science Foundation of China 21703255Research Fund for Scientific Research Level Improvement Plan of Anhui Medical University 2020xkjT001Research Fund of Anhui Institute of Translational Medicine 2021zhyx-C17Scientific Research Foundation for the Returned Overseas Chinese Scholars of Anhui Province-Key Projects 2021LCX003
6 · The paper itself

Abstract

A precisely designed dual-color biosensor has realized a visual assessment of thymidine kinase 1 (TK1) mRNA in both living cells and cell lysates. The oligonucleotide probe is constructed by hybridizing the antisense strand of the target and two recognition sequences, in which FAM serves as the donor and TAMRA as the acceptor. Once interacting with the target, two recognition strands are replaced, and then the antisense complementary sequence forms a more stable double-stranded structure. Due to the increasing spatial distance between two dyes, the FRET is attenuated, leading to a rapid recovery of FAM fluorescence and a reduction of TAMRA fluorescence. A discernible color response from orange to green could be observed by the naked eye, with a limit of detection (LOD) of 0.38 nM and 5.22 nM for spectrometer- and smartphone-based assays, respectively. The proposed ratiometric method transcends previous reports in its capacities in visualizing TK1 expression toward reliable nucleic acid biomarker analysis, which might establish a general strategy for ratiometric biosensing via strand displacement.

Indexed as

Fluorescence Resonance Energy TransferFluorescent DyesLimit of DetectionRNA, MessengerThymidine KinaseBiomarkersBiosensing TechniquesFluorometryHumansNucleic Acid HybridizationBiomarkersFluorescent DyesRNA, MessengerThymidine Kinasethymidine kinase 1Fluorescence resonance energy transferRatiometric fluorescent probeStrand displacementThymidine kinase 1

Identifiers

PMID38871953

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.