ArticleNature communications2024
Synthetic biodegradable microporous hydrogels for in vitro 3D culture of functional human bone cell networks.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Advances in 3D bioprinting of immunoregulatory biomaterials for bone regeneration.Materials today. Bio · 2026Review
- Machine learning-guided discovery of a bioactive hydrogel for spatiotemporally orchestrated tendon-to-bone healing.Bioactive materials · 2026Article
- hMSCs form Osteocyte-like Cell Networks within Strain-Stiffening Bottlebrush Polymer Hydrogels.bioRxiv : the preprint server for biology · 2026Article
- Adipose-mimetic granular hydrogels uncover biophysical cues driving breast cancer invasion.Cell biomaterials · 2026Article
- Biocompatible Cyclodextrin-Cannabinoid Agar-Xanthan Gum Hydrogels for Controlled Delivery and Antimicrobial Soft-Tissue Biomedical Applications.ACS omega · 2026Article
- Programmable Hydrogels for Surgical Interface Control: Function-Based Design, DNA-Based Molecular Modules, and Translational Evaluation.Gels (Basel, Switzerland) · 2026Review
- An agent-guided peptide hydrogel bio-stabilizer clamps pericellular viscoelastic drift.Nature communications · 2026Article
- Phase-separated hydrogels for advanced biomedical engineering: From material design to applications.Materials today. Bio · 2026Review
- Hydrogels for Bone Repair: Construction Strategies and Applications.Smart medicine · 2026Review
- Arresting Microphase Separation Encodes Material Mechanics by Sculpting Microarchitectures and Local Polymer Enrichment.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Cell flocculation and phase-separation support macro-scale tissue slab construction in a scaffold-free manner.Materials today. Bio · 2026Article
- 4D morphogenetic tissue engineering via gradient-crosslinked microporous hydrogel scaffolds.Materials today. Bio · 2026Article
- Ion‑Mediated Structural Engineering of Hydrogel Interfaces for Tunable Mechanical and Analyte Diffusion Properties in Electrochemical Biosensors.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Bone Organoids: A Novel Tool for Modeling and Managing Skeletal Disorders in Diabetes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Evaluation of the Physicochemical and Biological Properties of Calcium-Silicate-Based Root-End Filling Materials.Journal of functional biomaterials · 2026Article
- Recent advances in biomaterials for bone regeneration: Bridging innovation and clinical translation.Materials today. Bio · 2026Review
- Mechanically heterogeneous hydrogel with cell-programmed network restructuring promotes tissue regeneration by mechano-epigenetic modulation.Nature communications · 2026Article
- Collagen Type I as a Biological Barrier Interface in Biomimetic Microfluidic Devices: Properties, Applications, and Challenges.Biomimetics (Basel, Switzerland) · 2026Review
- Rethinking osteocyte modelsFrontiers in bioengineering and biotechnology · 2026Review
- Harnessing Advances in Bone Tissue Engineering for Design of Bone-on-Chip Systems.Advanced healthcare materials · 2026Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Generating 3D bone cell networks in vitro that mimic the dynamic process during early bone formation remains challenging. Here, we report a synthetic biodegradable microporous hydrogel for efficient formation of 3D networks from human primary cells, analysis of cell-secreted extracellular matrix (ECM) and microfluidic integration. Using polymerization-induced phase separation, we demonstrate dynamic in situ formation of microporosity (5-20 µm) within matrix metalloproteinase-degradable polyethylene glycol hydrogels in the presence of living cells. Pore formation is triggered by thiol-Michael-addition crosslinking of a viscous precursor solution supplemented with hyaluronic acid and dextran. The resulting microporous architecture can be fine-tuned by adjusting the concentration and molecular weight of dextran. After encapsulation in microporous hydrogels, human mesenchymal stromal cells and osteoblasts spread rapidly and form 3D networks within 24 hours. We demonstrate that matrix degradability controls cell-matrix remodeling, osteogenic differentiation, and deposition of ECM proteins such as collagen. Finally, we report microfluidic integration and proof-of-concept osteogenic differentiation of 3D cell networks under perfusion on chip. Altogether, this work introduces a synthetic microporous hydrogel to efficiently differentiate 3D human bone cell networks, facilitating future in vitro studies on early bone development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.