Generating Mutant for a Stress-Responsive Gene in Rice Using CRISPR-Cas9 System.

Chudamani Sharma Prakash, Ramanjulu Sunkar

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

2 authors.

Chudamani Sharma PrakashDepartment of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, USA.

Ramanjulu SunkarDepartment of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, USA. ramanjulu.sunkar@okstate.edu.

Funding

NSF-EPSCoR RII Track 2 FEC—1826836NSF Grant IOS-1849708

6 · The paper itself

Abstract

CRISPR/Cas9 system is one of the most often utilized engineering tools for genome editing in many organisms including crop plants and presents great value in both basic and applied research. This is a preferred method because of its relative simplicity, cost-effectiveness, and reliability. The Cas9 nuclease guided by a short single guide RNA (gRNA) can generate double-strand DNA breaks (DSB) at the specific sites in chromosomal DNA. The DSB site is repaired by error-prone repair methods. During repair, some nucleotides are deleted or added at the target site. Here, we present a simplified protocol for generating mutants in gene of interest in rice using CRISPR/Cas9.

Indexed as

CRISPR-Cas SystemsGene EditingOryzaRNA, Guide, CRISPR-Cas SystemsDNA Breaks, Double-StrandedMutationPlants, Genetically ModifiedStress, PhysiologicalRNA, Guide, CRISPR-Cas SystemsAgrobacteriumCas9Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)MutationTransformation

Identifiers

PMID38869803

DOI10.1071/FP05090

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.