Evidence map›Paper›PMID 38869797›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Reactive Oxygen Species (ROS) Measurement in Arabidopsis Guard Cells in Response to Biotic and Abiotic Stresses.

Shweta Chhajed, YangYang Li, Sixue Chen

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. PLETHORA-autophagy axis activates organ regeneration through ROS modulation.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Shweta ChhajedDepartment of Biology, University of Florida, Gainesville, FL, USA.
YangYang LiDepartment of Biology, University of Florida, Gainesville, FL, USA.
Sixue ChenDepartment of Biology, University of Florida, Gainesville, FL, USA. schen8@olemiss.edu.

Funding

Department of Agriculture 2020-67013-40658National Science Foundation 2340995
6 · The paper itself

Abstract

One of the major plant stress level indicators is reactive oxygen species (ROS). They have been known to play a central role in regulating plant responses to various environmental stresses. This book chapter specifically covers abiotic stress induced by a drought hormone abscisic acid and biotic stress induced by Pseudomonas syringe DC3000 on single cell-type guard cells. We describe in detail the measurement of ROS production starting from sample preparation to data analysis by fluorescence intensity acquisition using ImageJ software. We discussed the problems faced while performing the experiment and addressed how to overcome them by providing specific guidelines to ensure high quality repeatable data.

Indexed as

ArabidopsisReactive Oxygen SpeciesStress, PhysiologicalAbscisic AcidPseudomonas syringaeAbscisic AcidReactive Oxygen Species2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) stainingAbscisic acid (ABA)Guard cellsImageJ analysisPseudomonas syringae pv. tomato str. DC3000 (Pst DC3000)Reactive oxygen species (ROS)Single cell-type

Identifiers

PMID38869797

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.