Evidence map›Paper›PMID 38869792›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Validation of the Interaction Between Stress-Responsive LncRNAs and RNA-Binding Proteins In Vitro.

Liaoliao Ye, Tao Qin, Liming Xiong

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Liaoliao YeCollege of Grassland Agriculture, Northwest A&F University, Yangling, China.
Tao QinCollege of Grassland Agriculture, Northwest A&F University, Yangling, China.
Liming XiongDepartment of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. lxiong@hkbu.edu.hk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plant long non-coding RNAs (lncRNAs) have been implicated in many biological processes, including responses to abiotic stresses, yet their detailed functions and especially their modes of action are still underexplored. lncRNAs often interact with proteins to participate in multiple levels of gene regulation. Therefore, identifying the RNA-binding proteins and validating their interaction with lncRNAs will be instrumental in revealing the functions of lncRNAs. Here, we describe two major methods to determine the interaction between lncRNA and proteins in vitro, RNA pull-down, and RNA EMSA.

Indexed as

RNA-Binding ProteinsRNA, Long NoncodingElectrophoretic Mobility Shift AssayProtein BindingRNA, PlantStress, PhysiologicalRNA-Binding ProteinsRNA, Long NoncodingRNA, PlantLncRNARNA-binding proteinRNA EMSARNA pull-down

Identifiers

PMID38869792

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.