Evidence map›Paper›PMID 38869789›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

A Method to Quantitatively Examine Heat Stress-Induced Alternative Splicing in Plants by RNA-Seq and RT-PCR.

Yu Ling, Yujian Mo, Shanlan Chen, Magdy M Mahfouz

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yu LingCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, People's Republic of China.
Yujian MoCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, People's Republic of China.
Shanlan ChenCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, People's Republic of China.
Magdy M MahfouzLaboratory for Genome Engineering, Division of Biological Sciences, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia. magdy.mahfouz@kaust.edu.sa.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alternative splicing (AS) of pre-mRNAs is a type of post-transcriptional regulation in eukaryotes that expands the number of mRNA isoforms. Intron retention is the primary form of AS in plants and occurs more frequently when plants are exposed to environmental stresses. Several wet-lab and bioinformatics techniques are used to detect AS events, but these techniques are technically challenging or unsuitable for studying AS in plants. Here, we report a method that combines RNA-sequencing and reverse transcription PCR for visualizing and validating heat stress-induced AS events in plants, using Arabidopsis thaliana and HEAT SHOCK PROTEIN21 (HSP21) as examples.

Indexed as

Alternative SplicingArabidopsisHeat-Shock ResponseArabidopsis ProteinsComputational BiologyGene Expression Regulation, PlantReverse Transcriptase Polymerase Chain ReactionRNA, PlantRNA-SeqArabidopsis ProteinsRNA, PlantAlternative splicingBAM fileIGV programRNA sequencingRT-PCRSnapgeneStress

Identifiers

PMID38869789

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.