Evidence map›Paper›PMID 38869719›Full record

ArticleMikrochimica acta2024

Ultrasensitive dynamic light scattering immunodetection of alpha-fetoprotein using heptamer-amplified nanoparticle crosslinking aggregation.

Lu Ding, Jiaqi Hu, Xing Liu, Junyi Zeng, Zhiwen Hu, Jing Chen, Kang Zhu, Hong Duan, Xiaolin Huang

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lu Ding *Department of Cardiology, The First Affiliated Hospital, Jiangxi Medical College, Jiangxi Hypertension Research Institute, Nanchang University, Nanchang, 330006, P. R. China.
Jiaqi Hu *State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang, 330047, P. R. China.
Xing Liu *Key Laboratory of Tropical and Vegetables Quality and Safety for State Market Regulation, School of Food Science and Engineering, Hainan University, Haikou, 570228, P. R. China.
Junyi ZengDepartment of Cardiology, The First Affiliated Hospital, Jiangxi Medical College, Jiangxi Hypertension Research Institute, Nanchang University, Nanchang, 330006, P. R. China.
Zhiwen HuDepartment of Cardiology, The First Affiliated Hospital, Jiangxi Medical College, Jiangxi Hypertension Research Institute, Nanchang University, Nanchang, 330006, P. R. China.
Jing ChenState Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang, 330047, P. R. China.
Kang ZhuState Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang, 330047, P. R. China.
Hong DuanBeijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University, Beijing, 100048, P. R. China. duanhong202@163.com.
Xiaolin HuangState Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang, 330047, P. R. China. xiaolin.huang@ncu.edu.cn.ORCID http://orcid.org/0000-0002-8908-0443

Funding

Jiangxi Provincial Natural Science Foundation 20224ACB205012National Natural Science Foundation of China 32001788Scientific Research Foundation of Education Department of Anhui Province of China GJJ200221
6 · The paper itself

Abstract

A novel construction strategy is introduced for an ultrasensitive dynamic light scattering (DLS) immunosensor targeting alpha fetoprotein (AFP). This approach relies on a self-assembled heptamer fusion protein (A1-C4bpα), incorporating the dual functions of multivalent recognition and crosslinking aggregation amplification due to the presence of seven AFP-specific A1 nanobodies on the A1-C4bpα heptamer. Leveraging antibody-functionalized magnetic nanoparticles for target AFP capture and DLS signal output, the proposed heptamer-assisted DLS immunosensor offers high sensitivity, strong specificity, and ease of operation. Under the optimized conditions, the designed DLS immunosensor demonstrates excellent linear detection of AFP in the concentration range 0.06 ng mL

Indexed as

alpha-FetoproteinsDynamic Light ScatteringLimit of DetectionAntibodies, ImmobilizedBiosensing TechniquesHumansImmunoassayMagnetite NanoparticlesSingle-Domain AntibodiesAFP protein, humanalpha-FetoproteinsAntibodies, ImmobilizedMagnetite NanoparticlesSingle-Domain AntibodiesAlpha fetoproteinDynamic light scatteringFunctionalized magnetic nanoparticlesHeptamerImmunosensorNanobody

Identifiers

PMID38869719

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.