ArticleiMeta2023
MBPD: A multiple bacterial pathogen detection pipeline for One Health practices.
Article in iMeta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Seasonal dynamics of microbial communities and potential human pathogens inApplied and environmental microbiology · 2026Article
- Fecal Pathogenic Bacteria Composition and Community Assembly Mechanisms in Captive Brown Bears and Asiatic Black Bears.Animals : an open access journal from MDPI · 2026Article
- Mapping potential pathogen profiling in cetacean blow: comparative insights from sequencing technologies.Microbial genomics · 2026Article
- Multidimensional Differences and Driving Mechanisms of Bacterial Communities in Urban and Rural Rivers Across China.Microorganisms · 2026Article
- Potential Role of Captive Environments in Reshaping the Compositions of Pathogenic Gut Bacteria inBiology · 2026Article
- Article
- The Potential of Bioinformatics in Revolutionizing Disease Diagnosis in Sub-Saharan Africa: A Narrative Review.Health science reports · 2026Article
- Microbial succession accompanies increased antibiotic resistance risk during grass carp (Ctenopharyngodon idella) spoilage under ambient household conditions.BMC microbiology · 2026Article
- Soil antibiotic resistome in farmland exhibits higher diversity and horizontal transfer potential than adjacent pastureland in agro-pastoral ecotone.Environmental microbiome · 2026Article
- Temporal dynamics of the fecal microbiome in wintering seagulls: a One Health perspective.BMC genomics · 2026Article
- Uncovering Putative Bacterial Pathogens in Lakes in Aotearoa New Zealand Using Environmental DNA.Ecology and evolution · 2026Article
- Diversity and transmission processes of potentially pathogenic bacterial communities in the East Rongbuk Glaciers, Mt. Everest.Current research in microbial sciences · 2026Article
- Gut microbiome disruption in free-roaming cats: Do antibiotics reduce or restructure zoonotic risk?One health (Amsterdam, Netherlands) · 2025Article
- NEMESISdb: A full length 16S rRNA gene dataset for the detection of human, fish, and crustacean potentially pathogenic bacteria.Data in brief · 2025Article
- Discarded cigarette butts as overlooked reservoirs and amplifiers of antibiotic resistance genes and pathogens in urban green spaces.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Foot Traffic Driven Anthropogenic Activity Alters Phyllosphere Microbial Community Characteristics and Putative Pathogens in Subtropical Urban Green Spaces.Microorganisms · 2025Article
- Unstable pathogen profile in spotted seal (Phoca largha) gut microbiota and limited turnover with habitat microbiome.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- CeONPJ microgravity · 2025Article
- Molecular assessment of oyster microbiomes and viromes reveals their potential as pathogen and ecological sentinels.One health (Amsterdam, Netherlands) · 2025Article
- MVPCR: Multiplex Visual Detection Strategy Based on Ultrafast PCR for Point-of-Care Pathogens Detection Within 10 Min.Phenomics (Cham, Switzerland) · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial pathogens are one of the major threats to biosafety and environmental health, and advanced assessment is a prerequisite to combating bacterial pathogens. Currently, 16S rRNA gene sequencing is efficient in the open-view detection of bacterial pathogens. However, the taxonomic resolution and applicability of this method are limited by the domain-specific pathogen database, taxonomic profiling method, and sequencing target of 16S variable regions. Here, we present a pipeline of multiple bacterial pathogen detection (MBPD) to identify the animal, plant, and zoonotic pathogens. MBPD is based on a large, curated database of the full-length 16S genes of 1986 reported bacterial pathogen species covering 72,685 sequences. In silico comparison allowed MBPD to provide the appropriate similarity threshold for both full-length and variable-region sequencing platforms, while the subregion of V3-V4 (mean: 88.37%, accuracy rate compared to V1-V9) outperformed other variable regions in pathogen identification compared to full-length sequencing. Benchmarking on real data sets suggested the superiority of MBPD in a broader range of pathogen detections compared with other methods, including 16SPIP and MIP. Beyond detecting the known causal agent of animal, human, and plant diseases, MBPD is capable of identifying cocontaminating pathogens from biological and environmental samples. Overall, we provide a MBPD pipeline for agricultural, veterinary, medical, and environmental monitoring to achieve One Health.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.