ArticleiMeta2023
MultiPrime: A reliable and efficient tool for targeted next-generation sequencing.
Article in iMeta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Nucleosome-targeted host DNA depletion enables automated plasma metagenomic sequencing for sensitive detection of bloodstream pathogens.Journal of translational medicine · 2026Article
- Time-series dynamics and biocontrol potential of postharvest bacteria in litchi microbiota.Science China. Life sciences · 2026Article
- Identification and validation of a QTL, QSNS.caas-3D.1, for spikelet number per spike in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Advancing Plant Microbiome Research Through Host DNA Depletion Techniques.Plant biotechnology journal · 2026Review
- Review
- Accelerating primer design for amplicon sequencing using large language model-powered agents.Nature biomedical engineering · 2026Article
- Calibrating tetranucleotide-frequency distances for metagenomic binning with right-skewed distribution models.Bioinformatics advances · 2026Article
- Case Report: Torque teno virus identified in pleural effusion of pediatric severe Mycoplasma pneumoniae pneumonia: diagnostic and therapeutic implications.Frontiers in pediatrics · 2026Article
- The Function ofPlants (Basel, Switzerland) · 2025Article
- Elucidating the Molecular Mechanisms of Pulsed Light-Induced Lycopene Accumulation in Tomatoes Through Integrated Multi-Omics Analysis.International journal of molecular sciences · 2025Article
- EndoSCell: A portable intraoperative histopathological microscopy device for enhanced glioma resection and prognosis in animal models.APL bioengineering · 2025Article
- Identification and validation of immune-associated gene signatures for prognostic prediction in acute myeloid leukemia.Medicine · 2025Article
- Interferon in Liver Diseases: Recent Advances.Advances in therapy · 2025Review
- Application of targeted metagenomic next-generation sequencing in pneumonia patients.Microbiology spectrum · 2025Article
- Performance of broad-spectrum targeted next-generation sequencing in lower respiratory tract infections in ICU patients: a prospective observational study.Critical care (London, England) · 2025Observational
- Correlation and prognosis analysis of human papillomavirus infection and P16 expression in oral and oropharyngeal squamous cell carcinomas.Scientific reports · 2025Article
- A chromosome-level genome assembly and annotation of the Pseudorasbora elongata (Cypriniformes: Cyprinidae).Scientific data · 2025Article
- Enhancing Arthropod Diversity and Sorghum Quality in Northern Jiangsu, China: The Benefits of Green Pest Management Revealed Through Metabarcoding.International journal of molecular sciences · 2025Article
- Article
- Clinical diagnosis of Q fever by targeted next-generation sequencing for identification of Coxiella burnetii.BMC infectious diseases · 2025Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next-generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime-designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.