ArticleiMeta2023
Primer selection impacts the evaluation of microecological patterns in environmental microbiomes.
Article in iMeta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Dual-platform metagenomic surveillance distinguishes pathogen and resistome hotspots across agricultural and mixed-use watersheds.One health (Amsterdam, Netherlands) · 2026Article
- Unleashing the potential of mRNA-seq to uncover the microbiome structure and their crosstalk with host cells: the vulvar ecosystem.Microbiome · 2026Article
- Optimizing primer selection for microbial diversity studies in freshwater lakes: a comprehensive evaluation of 16S rRNA, 18S rRNA, and ITS gene regions.FEMS microbiology ecology · 2026Article
- Nutrient content of grass influences microbial communities in the phyllosphere more than climate change.Applied microbiology and biotechnology · 2026Article
- An exploratory investigation into the microbial and cyanobacterial presence on skin epibiotia and orofacial lesions in estuarine common bottlenose dolphins (Tursiops truncatus) through metabarcoding.Scientific reports · 2026Article
- Multi-Habitat Landscape Promotes Microbial Diversity: Insights from the Traditional Agricultural Heritage and the Global Trend.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Evaluating the role of microplastics and wastewater in shaping Vibrio spp. and antibiotic resistance gene abundance in urban freshwaters.Scientific reports · 2025Article
- Asymbiotic Nitrogen Fixation in the Phyllosphere of Urban Green Spaces.Current microbiology · 2025Article
- Pathogenicity and host-interacting mechanisms of enterogenicFrontiers in microbiology · 2025Article
- Fitness effects of synthetic and natural diet preservatives on the edible insect Bombyx mori.NPJ science of food · 2024Article
- Article
- Rhizobacterial diversity, community composition, and the influence of keystone taxa on O'Neal blueberry (Frontiers in microbiology · 2024Article
- Review
- Primer selection impacts the evaluation of microecological patterns in environmental microbiomes.iMeta · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study revealed that primer selection substantially influences the taxonomic and predicted functional composition and the characterization of microecological patterns, which was not alleviated by close-reference clustering. Biases were relatively consistent across different habitats in community profiling but not in microecological patterns. These primer biases could be attributed to multiple aspects, including taxa specificity, regional hypervariability, and amplification efficiency.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.