ArticleiMeta2023
Cultivation strategies for prokaryotes from extreme environments.
Article in iMeta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Harnessing synthetic microbial communities for desert vegetation restoration.Synthetic and systems biotechnology · 2027Review
- Article
- Omics-Guided Construction of Microbial Consortia for Reproducible Traditional Fermented Foods and Beverages.Foods (Basel, Switzerland) · 2026Review
- Decoding extremophiles: insights from bioinformatics, machine learning, and data-driven approaches.Briefings in bioinformatics · 2026Review
- The Extreme Environment Microbiome Catalog (EEMC): a global resource for microbial diversity and antimicrobial discovery.Nature communications · 2026Article
- Microbial decomposer diversity and metabolic function during the decomposition of brine shrimp carcasses in a saline lake.Microbiome · 2026Article
- Bacterial Siderophore Production in Metal-Rich Environments: Underexplored Sources of Siderophores and Insights into Bioremediation.Journal of natural products · 2026Review
- Variations in deep-sea microbial composition and assembly mechanisms under different culture strategies.Frontiers in microbiology · 2026Article
- iHalo: a genome-based machine learning model using k-mer for halophiles identification.World journal of microbiology & biotechnology · 2025Article
- Brevibacillus nigriterrae sp. nov., a Novel Denitrification Bacterium Isolated from Black Soil.Current microbiology · 2025Article
- Article
- Enzymatic potential of dairy microbes from high-altitude niches: emerging resource with industrial applications.Extremophiles : life under extreme conditions · 2025Review
- The Occurrence ofJournal of fungi (Basel, Switzerland) · 2025Article
- Review
- Temperature-Sensitive Lipids Reveal Intraspecific Diversity in Bacteria Isolated from an Ancient Antarctic Microbial Mat.Microbial ecology · 2025Article
- Trujillonella humicola sp. nov., a siderophore-synthesizing bacterium isolated from black soil in Northeast China.Antonie van Leeuwenhoek · 2025Article
- Discovery and cultivation of prokaryotic taxa in the age of metagenomics and artificial intelligence.The ISME journal · 2025Article
- The familyFrontiers in microbiology · 2025Review
- Insights into chemoautotrophic traits of a prevalent bacterial phylum CSP1-3, hereinNational science review · 2024Article
- Roseobacter sinensis sp. nov., a marine bacterium capable to synthesize arachidonic acid.Antonie van Leeuwenhoek · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The great majority of microorganisms are as-yet-uncultivated, mostly found in extreme environments. High-throughput sequencing provides data-rich genomes from single-cell and metagenomic techniques, which has enabled researchers to obtain a glimpse of the unexpected genetic diversity of "microbial dark matter." However, cultivating microorganisms from extreme environments remains essential for dissecting and utilizing the functions of extremophiles. Here, we provide a straightforward protocol for efficiently isolating prokaryotic microorganisms from different extreme habitats (thermal, xeric, saline, alkaline, acidic, and cryogenic environments), which was established through previous successful work and our long-term experience in extremophile resource mining. We propose common processes for extremophile isolation at first and then summarize multiple cultivation strategies for recovering prokaryotic microorganisms from extreme environments and meanwhile provide specific isolation tips that are always overlooked but important. Furthermore, we propose the use of multi-omics-guided microbial cultivation approaches for culturing these as-yet-uncultivated microorganisms and two examples are provided to introduce how these approaches work. In summary, the protocol allows researchers to significantly improve the isolation efficiency of pure cultures and novel taxa, which therefore paves the way for the protection and utilization of microbial resources from extreme environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.