ArticleMicrobial cell factories2024
Transforming microbial pigment into therapeutic revelation: extraction and characterization of pyocyanin from Pseudomonas aeruginosa and its therapeutic potential as an antibacterial and anticancer agent.
Article in Microbial cell factories, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Bacterial Pigments as Potential Antitumor Agents Against Gastrointestinal Cancers: A Comprehensive Systematic Review.Biotechnology and applied biochemistry · 2026Pooled it
- Analytical characterization, antifungal activity and textile dyeing of blue green pigment produced by Pseudomonas aeruginosa for potential industrial application.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Modular integration of nanopore sequencing, alphafold modeling, and statistical design boosts 1-hydroxyphenazine yield in Pseudomonas aeruginosa strain KAEH25.BMC microbiology · 2026Article
- Optimization of Pyocyanin Production byAntibiotics (Basel, Switzerland) · 2026Article
- Microbial pigments as potential anti-rift valley fever virus drugs.BMC chemistry · 2026Article
- Development of MWCNTs/MXene/PVA Hydrogel Electrochemical Sensor for Multiplex Detection of Wound Infection Biomarkers.Micromachines · 2026Article
- Anticancer and immune effect of the extracted brownOpen veterinary journal · 2026Article
- Enhanced Production of Antimicrobial Pyocyanin Using Electromagnetic Fields and Carbon Nanotubes.Nanotechnology, science and applications · 2026Article
- Blue-green pigment production by environmental isolates of Pseudomonas aeruginosa: Purification and bioactivity.Folia microbiologica · 2025Article
- An insight into pyocyanin: production, characterization, and evaluation of its in vitro antibacterial, antifungal, antibiofilm and in vivo anti-schistosomal potency.BMC microbiology · 2025Article
- Multi-walled carbon nanotubes as reusable boosters of pyocyanin production for anticancer research.Applied microbiology and biotechnology · 2025Article
- Prospects of Synthetic Biology-Based Approaches in the Enhanced Production of Pyocyanin in Pseudomonas aeruginosa MCCB 117 as the Drug of Choice in Aquaculture.Current microbiology · 2025Review
- Nutritional optimization for bioprocess production of staphyloxanthin from Staphylococcus aureus with response surface methodology: promising anticancer scaffold targeting EGFR inhibition.Microbial cell factories · 2025Article
- Impact of heterologous expression of Cannabis sativa tetraketide synthase on Phaeodactylum tricornutum metabolic profile.Biotechnology for biofuels and bioproducts · 2025Article
- Review
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe objectives of the current study were to extract pyocyanin from Pseudomonas aeruginosa clinical isolates, characterize its chemical nature, and assess its biological activity against different bacteria and cancer cells. Due to its diverse bioactive properties, pyocyanin, being one of the virulence factors of P. aeruginosa, holds a promising, safe, and available therapeutic potential.
methods30 clinical P. aeruginosa isolates were collected from different sources of infections and identified by routine methods, the VITEK 2 compact system, and 16 S rRNA. The phenazine-modifying genes (phzM, phzS) were identified using polymerase chain reaction (PCR). Pyocyanin chemical characterization included UV-Vis spectrophotometry, Fourier Transform Infra-Red spectroscopy (FTIR), Gas Chromatography-Mass Spectrometry (GC-MS), and Liquid Chromatography-Mass Spectrometry (LC-MS). The biological activity of pyocyanin was explored by determining the MIC values against different clinical bacterial strains and assessing its anticancer activity against A549, MDA-MB-231, and Caco-2 cancer cell lines using cytotoxicity, wound healing and colony forming assays.
resultsAll identified isolates harboured at least one of the phzM or phzS genes. The co-presence of both genes was demonstrated in 13 isolates. The UV-VIS absorbance peaks were maxima at 215, 265, 385, and 520 nm. FTIR could identify the characteristic pyocyanin functional groups, whereas both GC-MS and LC-MS elucidated the chemical formula C
conclusionsThe extracted pyocyanin has demonstrated to be a potentially effective candidate against various bacterial infections and cancers. Hence, the current findings could contribute to producing this natural compound easily through an affordable method. Nonetheless, future studies are required to investigate pyocyanin's effects in vivo and analyse the results of combining it with other traditional antibiotics or anticancer drugs.
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